A Comparative Study on Estimation of Total Phenolic Content and Antioxidant Activity of Selected Medicinal Plants

Abstract:

Medicinal plants are a rich source of bioactive compounds, particularly phenolic constituents, which play a crucial role in combating oxidative stress through their antioxidant properties. The present study focuses on a comparative evaluation of total phenolic content (TPC) and antioxidant activity in selected medicinal plants widely used in traditional medicine. Plant extracts were prepared using suitable solvents, and the TPC was quantified using the Folin–Ciocalteu method, expressed as gallic acid equivalents (GAE). Antioxidant activity was assessed through standard in vitro assays such as DPPH free radical scavenging, ABTS assay, and reducing power capacity. The results revealed significant variations in phenolic content and antioxidant potential among the studied plant species, indicating that phytochemical composition is strongly influenced by plant type and extraction method. Plants exhibiting higher phenolic content demonstrated stronger free radical scavenging activity, suggesting a positive correlation between phenolic compounds and antioxidant efficiency. Statistical analysis further supported the relationship between TPC and antioxidant assays, confirming the role of phenolics as primary contributors to antioxidant activity. This comparative study highlights the importance of medicinal plants as natural antioxidants and supports their potential application in pharmaceutical and nutraceutical formulations. The findings also provide a scientific basis for the traditional use of these plants in managing oxidative stress-related disorders. Further studies focusing on isolation and characterization of specific phenolic compounds may enhance the development of plant-based therapeutic agents.