Published May 26, 2026 | Version 1
Dataset Open

Ageing blunts the phospho-proteomic response to resistance exercise in humans - data and code

Authors/Creators

  • 1. School of Sport Exercise and Rehabilitation, University of Technology Sydney, Sydney, NSW, Australia.
  • 2. Centenary Institute, Sydney, NSW, Australia.
  • 3. Department of Physiology, Nutrition and Biomechanics, Astrand Laboratory, Swedish School of Sport and Health Sciences, Stockholm, Sweden.
  • 4. Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC, Australia.
  • 5. reNEW, Novo Nordisk Foundation Centre for Stem Cell Medicine, Murdoch Children's Research Institute, Melbourne, VIC, Australia.
  • 6. Department of Women's and Children's Health, Karolinska Institute, Stockholm, Sweden.
  • 7. Department of Paediatrics, University of Melbourne, Melbourne, Victoria Australia.
  • 8. Department of Kinesiology, Health Promotion & Recreation, University of North Texas, Texas, USA.
  • 9. Division of Cardiovascular Sciences, University of Leicester, Leicester, UK.
  • 10. NIHR Leicester Biomedical Research Centre, University of Leicester, Leicester, UK.
  • 11. Australian Regenerative Medicine Institute, Monash University, Clayton, Australia.

Description

Ageing blunts the adaptive growth response in human skeletal muscle. To define the molecular processes underlying this impairment, we performed unbiased analysis of the phosphoproteome and total proteome in healthy young and old human skeletal muscle following acute resistance exercise (ResEX) and essential amino acid (EAA) ingestion. Ageing led to global suppression of the growth-induced global phosphoproteome, despite intact mTORC1 activation in old muscle. Our results identify widespread effects of ageing on skeletal muscle growth and highlight novel pathways for therapeutic development
in aged skeletal muscle.

 

This repository contains the code and data associated with the paper Ageing blunts the phospho-proteomic response to resistance exercise in humans. https://doi.org/10.64898/2026.06.02.729449

The zip file contains:

  • Processed phosphoproteome and proteome data sets (including missing and imputed values matrices)
  • Differential analysis results.
  • Rokai analysis results.
  • Functional geneset enrichment analysis reactome results.
  • Code for analysis and creating plots and figure panels.

 

Files

ageing-blunts-the-phospho-proteomic-response-data-and-code.zip

Files (95.2 MB)