Data and R code: Sublethal effects of the organosilicone surfactant Silwet L-77 and its interactions with acetamiprid and sulfoxaflor on honeybee (Apis mellifera) gustatory responsiveness and olfactory learning

Description of the data and file structure

This README provides information about the data files associated with the manuscript titled “Sublethal effects of the organosilicone surfactant Silwet L-77 and its interactions with acetamiprid and sulfoxaflor on honeybee (Apis mellifera) gustatory responsiveness and olfactory learning” published in Environmental Pollution (doi: 10.1016/j.envpol.2026.129380). These files contain the entire dataset and RMD script on which our statistical analysis was based on. 

Files and variables

File: Achatz_etal_2026_Data.csv

Description: This is the main data file, containing:

Bee = bee ID

Group = each day collected bees either assigned an early (1) or later (2; experimental procedure started 75 min delayed) 

Date = date (yyyy-mm-dd) of assessment

Treatment_group = treatment group to which bees were assigned (combined inforamtion about treatment and dose)

Treatment = treatment to which bees were assigned

Dose_SIL= treatment dose of Silwet L-77 (in %)

Dose_SFX = treatment dose of Sulfoxaflor (in ng)

Dose_ACE = treatment dose of Acetamiprid (in ng)

GRS_0 = gustatory response to 0% sucrose, i.e. pure tap water (0 = no response, 1 = responed)

GRS_0.1 = gustatory response to 0.1% sucrose (0 = no response, 1 = responed)

GRS_0.3 = gustatory response to 0.3% sucrose (0 = no response, 1 = responed)

GRS_1 = gustatory response to 1% sucrose (0 = no response, 1 = responed)

GRS_3 = gustatory response to 3% sucrose (0 = no response, 1 = responed)

GRS_10 = gustatory response to 10% sucrose (0 = no response, 1 = responed)

GRS_30 = gustatory response to 30% sucrose (0 = no response, 1 = responed)

Air_1 = PER to air flow before aquisition trial 1 (0 = no response, 1 = responed)

Trial_1 = PER to hexanal during aquisition trial 1 (0 = no response, 1 = responed)

Sucorse_1 = PER to 30% sucrose solution during aquisition trial 1 (0 = no response, 1 = responed)

Air_2 = PER to air flow before aquisition trial 2 (0 = no response, 1 = responed)

Trial_2 = PER to hexanal during aquisition trial 2 (0 = no response, 1 = responed)

Sucorse_2 = PER to 30% sucrose solution during aquisition trial 2 (0 = no response, 1 = responed)

Air_3 = PER to air flow before aquisition trial 3 (0 = no response, 1 = responed)

Trial_3 = PER to hexanal during aquisition trial 3 (0 = no response, 1 = responed)

Sucorse_3 = PER to 30% sucrose solution during aquisition trial 3 (0 = no response, 1 = responed)

Air_4 = PER to air flow before aquisition trial 4 (0 = no response, 1 = responed)

Trial_4 = PER to hexanal during aquisition trial 4 (0 = no response, 1 = responed)

Sucorse_4 = PER to 30% sucrose solution during aquisition trial 4 (0 = no response, 1 = responed)

Air_5 = PER to air flow before aquisition trial 5 (0 = no response, 1 = responed)

Trial_5 = PER to hexanal during aquisition trial 5 (0 = no response, 1 = responed)

Sucorse_5 = PER to 30% sucrose solution during aquisition trial 5 (0 = no response, 1 = responed)

Memory_90 = PER to hexanal during memory retention trial after 90 min (0 = no response, 1 = responed)

Died = any bee that died during the experiment (0 = did not die, 1 = died)

Notes = any addtional notes during the experiment

File: Achatz_etal_2026_Stats.Rmd

Description: This is the R Markdown file containing the complete R code from data tidying to reproducing the statistical analysis and figures presented in our manuscript. 

File: Achatz_etal_2026_Stats.pdf

Description: This is the R Markdown output containing the complete R code and output of the statistical analysis and figures presented in our manuscript.