Published February 8, 2024
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Figure 7 in Galactomannan inhibits Trichinella spiralis invasion of intestinal epithelium cells and enhances antibody-dependent cellular cytotoxicity related killing of larvae by driving macrophage polarization
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Figure 7. mRNA expression level of M1/M2 genes in RAW264.7 macrophages. The mRNA expression of M1/M2 cytokines in RAW264.7 macrophages treated with rTsgal or GM was analyzed by qPCR. After treatment with 20 µg/mL rTsgal or 1.6% GM at 37 °C for 24 h, the transcription level of M1 cytokines (IL-1β, IL-6 and TNF-α) was clearly increased in RAW264.7 macrophages, whereas the transcription level of M2 cytokines (IL-10, TGF-β) and surface marker CD206 was not evidently changed. The mRNA expression levels of the cytokines were calculated with the Ct (2—ΔΔCt) method. GAPDH was utilized as an internal control. *p <0.0001 compared to the DMEM group.
Notes
Published as part of Zhang, Ru, Zhang, Yao, Yan, Shu Wei, Cheng, Yong Kang, Zheng, Wen Wen, Long, Shao Rong, Wang, Zhong Quan & Cui, Jing, 2024, Galactomannan inhibits Trichinella spiralis invasion of intestinal epithelium cells and enhances antibody-dependent cellular cytotoxicity related killing of larvae by driving macrophage polarization, pp. 1-16 in Parasite (Paris, France) 31 (6) on page 9, DOI: 10.1051/parasite/2024002, http://zenodo.org/record/20228412
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- Journal article: 10.1051/parasite/2024002 (DOI)
- Journal article: urn:lsid:plazi.org:pub:7D68FFF0FFF17A3EFFE6FFC6845EFFA9 (LSID)
- Journal article: http://publication.plazi.org/id/7D68FFF0FFF17A3EFFE6FFC6845EFFA9 (URL)
- Journal article: https://zenodo.org/record/20228412 (URL)