Published December 8, 2025 | Version 2
Journal article Open

Leaf chamber experiments on sunflowers indicate a temperature-dependent compensation point of carbonyl sulfide

  • 1. Meteorology and Air Quality, Wageningen University & Research, Wageningen, Gelderland, The Netherlands
  • 2. Centre for Crop Systems Analysis, Wageningen University & Research, Wageningen, Gelderland, The Netherlands
  • 3. Horticulture and Product Physiology, Wageningen University & Research, Wageningen, Gelderland, The Netherlands
  • 4. Copernicus Institute of Sustainable Development, Utrecht University, Utrecht, Utrecht, The Netherlands
  • 5. Institute for Marine and Atmospheric Research, Utrecht University, Utrecht, The Netherlands

Description

Carbonyl sulfide (COS) is a trace gas found in the atmosphere that plants take up in a way similar to carbon dioxide (CO 2) during photosynthesis. Because of this shared pathway, COS has been widely used to estimate how much CO 2 plants absorb from the atmosphere—a process known as gross primary production (GPP). In our study, we conducted experiments using sunflower leaves in controlled chambers and found that COS and CO 2 respond differently to changes in temperature and humidity. Surprisingly, we also observed that sunflower leaves may release COS back into the atmosphere when COS levels are low and temperatures are high. This finding challenges the long-standing assumption that plants only absorb COS and do not emit it. Our research highlights that understanding how COS behaves in plants under different environmental conditions is crucial for using it as a reliable tool to estimate how much CO 2 is absorbed by vegetation.

Files

openreseurope-5-23939.pdf

Files (4.4 MB)

Name Size Download all
md5:1f6a151608a5f85e9323a5fb872c112c
4.4 MB Preview Download

Additional details

References

  • Adnew GA, Hofmann ME, Pons TL (2021). Leaf scale quantification of the effect of photosynthetic gas exchange on ∆ of CO . Sci Rep. doi:10.1038/s41598-021-93092-0
  • Adnew GA, Pons TL, Koren G (2020). Leaf-scale quantification of the effect of photosynthetic gas exchange on O of atmospheric CO . Biogeosciences. doi:10.5194/bg-17-3903-2020
  • Adnew GA, Pons TL, Koren G (2023). Exploring the potential of ∆ O in CO for determining mesophyll conductance. Plant Physiol. doi:10.1093/plphys/kiad173
  • Asaf D, Rotenberg E, Tatarinov F (2013). Ecosystem photosynthesis inferred from measurements of carbonyl sulphide flux. Nat Geosci. doi:10.1038/NGEO1730
  • Badger M, Collatz G (1977). Studies on the kinetic mechanism of ribulose-1,5-bisphosphate carboxylase and oxygenase reactions with particular reference to the effect of temperature on kinetic parameters. Carnegie Institution of Washington Yearbook,.
  • Belviso S, Abadie C, Montagne D (2022). Carbonyl Sulfide (COS) emissions in two agroecosystems in central France. PLoS One. doi:10.1371/journal.pone.0278584
  • Berry J, Wolf A, Campbell JE (2013). A coupled model of the global cycles of carbonyl sulfide and CO : a possible new window on the carbon cycle. JGR: Biogeosciences. doi:10.1002/jgrg.20068
  • Black VJ, Jones HG (1985). Plants and microclimate. J Appl Ecol.
  • Bonan GB (2008). Forests and climate change: forcings, feedbacks, and the climate benefits of forests. Science. doi:10.1126/science.1155121
  • Bosman PJ, Krol MC (2023). ICLASS 1.1, a variational Inverse modelling framework for the Chemistry land-surface atmosphere soil slab model: description, validation, and application. Geosci Model Dev. doi:10.5194/gmd-16-47-2023
  • Brasseur GP, Jacob DJ (2017). Modeling of atmospheric chemistry. doi:10.1017/9781316544754
  • Campbell JE, Carmichael GR, Chai T (2008). Photosynthetic control of atmospheric carbonyl sulfide during the growing season. Science. doi:10.1126/science.1164015
  • Cernusak LA, Ubierna N, Jenkins MW (2018). Unsaturation of vapour pressure inside leaves of two conifer species. Sci Rep. doi:10.1038/s41598-018-25838-2
  • Chevallier F, Bréon FM, Rayner PJ (2007). Contribution of the Orbiting Carbon Observatory to the estimation of CO sources and sinks: theoretical study in a variational data assimilation framework. J Geophys Res Atmos. doi:10.1029/2006JD007375
  • Cho A, Kooijmans LMJ, Driever SM (2025). COS_sunflower: dataset and software for : leaf chamber experiments on sunflowers indicate a temperature (v1.0). Zenodo.
  • Cho A, Kooijmans LM, Kohonen KM (2023). Optimizing the carbonic anhydrase temperature response and stomatal conductance of carbonyl sulfide leaf uptake in the Simple Biosphere model (SiB4). Biogeosciences. doi:10.5194/bg-20-2573-2023
  • Cochavi A, Amer M, Stern R (2021). Differential responses to two heatwave intensities in a mediterranean citrus orchard are identified by combining measurements of fluorescence and carbonyl Sulfide (COS) and CO uptake. New Phytol. doi:10.1111/nph.17247
  • Collatz GJ, Ball JT, Grivet C (1991). Physiological and environmental regulation of stomatal conductance, photosynthesis and transpiration: a model that includes a laminar boundary layer. Agric For Meteorol. doi:10.1016/0168-1923(91)90002-8
  • Daniel RM, Peterson ME, Danson MJ (2010). The molecular basis of the effect of temperature on enzyme activity. Biochem J. doi:10.1042/BJ20091254
  • Farquhar GD, Sharkey TD (1982). Stomatal conductance and photosynthesis. Annu Rev Plant Physiol. doi:10.1146/annurev.pp.33.060182.001533
  • Farquhar GD, von Caemmerer S, Berry JA (1980). A biochemical model of photosynthetic CO assimilation in leaves of C species. Planta. doi:10.1007/BF00386231
  • Garen JC, Branch HA, Borrego I (2022). Gas exchange analysers exhibit large measurement error driven by internal thermal gradients. New Phytol. doi:10.1111/nph.18347
  • Geng C, Mu Y (2004). Carbonyl sulfide and dimethyl sulfide exchange between lawn and the atmosphere. J Geophys Res D: Atmos. doi:10.1029/2003JD004492
  • Gimeno TE, Ogée J, Royles J (2017). Bryophyte gas-exchange dynamics along varying hydration status reveal a significant Carbonyl Sulphide (COS) sink in the dark and COS source in the light. New Phytol. doi:10.1111/nph.14584
  • Goldan PD, Fall R, Kuster WC (1988). Uptake of COS by growing vegetation: a major tropospheric sink. J Geophys Res. doi:10.1029/JD093iD11p14186
  • Hussain SB, Stinziano J, Pierre MO (2024). Accurate photosynthetic parameter estimation at low stomatal conductance: effects of cuticular conductance and instrumental noise. Photosynth Res. doi:10.1007/s11120-024-01092-8
  • Jarman PD (1974). The diffusion of carbon dioxide and water vapour through stomata. J Exp Bot. doi:10.1093/jxb/25.5.927
  • Kesselmeier J, Merk L (1993). Exchange of Carbonyl Sulfide (COS) between agricultural plants and the atmosphere: Studies on the deposition of COS to peas, corn and rapeseed. Biogeochemistry. doi:10.1007/BF00002922
  • Kettle AJ, Kuhn U, Von Hobe M (2002). Global budget of atmospheric Carbonyl Sulfide: temporal and spatial variations of the dominant sources and sinks. J Geophys Res. doi:10.1029/2002JD002187
  • Kohonen KM, Dewar R, Tramontana G (2022). Intercomparison of methods to estimate gross primary production based on CO and COS flux measurements. Biogeosciences. doi:10.5194/bg-19-4067-2022
  • Kooijmans LM, Cho A, Ma J (2021). Evaluation of Carbonyl Sulfide biosphere exchange in the Simple Biosphere Model (SiB4). Biogeosciences. doi:10.5194/bg-18-6547-2021
  • Kooijmans LMJ, Sun W, Aalto J (2019). Influences of light and humidity on Carbonyl Sulfide-based estimates of photosynthesis. Proc Natl Acad Sci U S A. doi:10.1073/pnas.1807600116
  • Kooijmans LMJ, Uitslag NAM, Zahniser MS (2016). Continuous and high-precision atmospheric concentration measurements of COS, CO , CO and H O using a Quantum Cascade Laser Spectrometer (QCLS). Atmos Meas Tech. doi:10.5194/amt-9-5293-2016
  • Kuhn U, Kesselmeier J (2000). Environmental variables controlling the uptake of Carbonyl Sulfide by lichens. J Geophys Res. doi:10.1029/2000JD900436
  • Lai J, Kooijmans LMJ, Sun W (2024). Terrestrial photosynthesis inferred from plant carbonyl sulfide uptake. Nature. doi:10.1038/s41586-024-08050-3
  • Le Roux X, Bariac T, Sinoquet H (2001). Spatial distribution of leaf water-use efficiency and carbon isotope discrimination within an isolated tree crown. Plant, Cell and Environment. doi:10.1046/j.0016-8025.2001.00756.x
  • Lobo FdA, de Barros MP, Dalmagro HJ (2013). Fitting net photosynthetic light-response curves with - a critical look at the models. Photosynthetica. doi:10.1007/s11099-013-0045-y
  • Lorimer GH, Pierce J (1989). Carbonyl sulfide: an alternate substrate for but not an activator of ribulose-1,5-bisphosphate carboxylase. J Biol Chem. doi:10.1016/S0021-9258(19)81679-4
  • Maseyk K, Berry JA, Billesbach D (2014). Sources and sinks of Carbonyl Sulfide in an agricultural field in the Southern Great Plains. Proc Natl Acad Sci U S A. doi:10.1073/pnas.1319132111
  • Montzka SA, Calvert P, Hall BD (2007). On the global distribution, seasonality, and budget of atmospheric Carbonyl Sulfide (COS) and some similarities to CO . J Geophys Res. doi:10.1029/2006JD007665
  • Notni J, Schenk S, Protoschill-Krebs G (2007). The missing link in COS metabolism: a model study on the reactivation of carbonic anhydrase from its hydrosulfide analogue. ChemBioChem. doi:10.1002/cbic.200600436
  • Ogée J, Sauze J, Kesselmeier J (2016). A new mechanistic framework to predict OCS fluxes from soils. Biogeosciences. doi:10.5194/bg-13-2221-2016
  • Protoschill-Krebs G, Kesselmeier J (1992). Enzymatic pathways for the Consumption of Carbonyl Sulphide (COS) by higher plants. Bot Acta. doi:10.1111/j.1438-8677.1992.tb00288.x
  • Protoschill-Krebs G, Wilhelm C, Kesselmeier J (1996). Consumption of Carbonyl Sulphide (COS) by higher plant Carbonic Anhydrase (CA). Atmos Environ. doi:10.1016/1352-2310(96)00026-X
  • Reichstein M, Falge E, Baldocchi D (2005). On the separation of net ecosystem exchange into assimilation and ecosystem respiration: review and improved algorithm. Glob Chang Biol. doi:10.1111/j.1365-2486.2005.001002.x
  • Rennenberg H (1991). The significance of higher plants in the emission of sulfur compounds from Terrestrial Ecosystems.
  • Salvucci ME, Osteryoung KW, Crafts-Brandner SJ (2001). Exceptional sensitivity of Rubisco activase to thermal denaturation and . Plant Physiol. doi:10.1104/pp.010357
  • Sandoval-Soto L, Stanimirov M, von Hobe M (2005). Global uptake of Carbonyl Sulfide (COS) by terrestrial vegetation: estimates corrected by deposition velocities normalized to the uptake of carbon dioxide (CO ). Biogeosciences. doi:10.5194/bg-2-125-2005
  • Seibt U, Kesselmeier J, Sandoval-Soto L (2010). A kinetic analysis of leaf uptake of COS and its relation to transpiration, photosynthesis and carbon isotope fractionation. Biogeosciences. doi:10.5194/bg-7-333-2010
  • Stimler K, Berry JA, Montzka SA (2011). Association between Carbonyl Sulfide uptake and ∆ during gas exchange in C and C leaves. Plant Physiol. doi:10.1104/pp.111.176578
  • Stimler K, Montzka SA, Berry JA (2010). Relationships between Carbonyl Sulfide (COS) and CO during leaf gas exchange. New Phytol. doi:10.1111/j.1469-8137.2010.03218.x
  • Sun W, Kooijmans LM, Maseyk K (2018). Soil fluxes of Carbonyl Sulfide (COS), carbon monoxide, and carbon dioxide in a boreal forest in southern Finland. Atmos Chem Phys. doi:10.5194/acp-18-1363-2018
  • Sun W, Maseyk K, Lett C (2015). A soil diffusion-reaction model for surface COS flux: COSSM v1. Geosci Model Dev. doi:10.5194/gmd-8-3055-2015
  • Tanner CB, Sinclair TR (2015). Efficient water use in crop production: research or re-search?. doi:10.2134/1983.limitationstoefficientwateruse.c1
  • Tarantola A (2005). Inverse problem theory and methods for model parameter estimation. doi:10.1137/1.9780898717921
  • Tcherkez GG, Farquhar GD, Andrews TJ (2006). Despite slow catalysis and confused substrate specificity, all ribulose bisphosphate carboxylases may be nearly perfectly optimized. Proc Natl Acad Sci U S A. doi:10.1073/pnas.0600605103
  • von Caemmerer S, Evans JR (2015). Temperature responses of mesophyll conductance differ greatly between species. Plant Cell Environ. doi:10.1111/pce.12449
  • von Caemmerer S, Farquhar GD (1981). Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves. Planta. doi:10.1007/BF00384257
  • Williams TG, Flanagan LB (1996). Effect of changes in water content on photosynthesis, transpiration and discrimination against CO and C O O in and . Oecologia. doi:10.1007/BF00333212
  • Wohlfahrt G, Gerdel K, Migliavacca M (2018). Sun-induced fluorescence and gross primary productivity during a heat wave. Sci Rep. doi:10.1038/s41598-018-32602-z
  • Wohlfahrt G, Gu L (2015). The many meanings of gross photosynthesis and their implication for photosynthesis research from leaf to globe. Plant Cell Environ. doi:10.1111/pce.12569
  • Wong SC, Canny MJ, Holloway-Phillips M (2022). Humidity gradients in the air spaces of leaves. Nat Plants. doi:10.1038/s41477-022-01202-1