From Microscale to Nanoscale Shadow Electrochemiluminescence Microscopy

The present dataset includes data generated as part of the 2PhotonECL, focusing on the imaging limit of shadow electrochemiluminescence (SECL).

Content of the folders:
• folder "decrease ratio" contains data regarding:
 - the SECL raw images used to extract the ECL intensity (a.u.) for calculating decrease SECL ratio recorded in the Procell solution contained 30 µM [Ru(bpy)3]2+ on GCE or 0.1 M PBS (pH = 7.4) containing 1 mM [Ru(bpy)3]2+ and 100 mM TPA on ITO.
 - the decrease SECL ratio of different size of sperichal targets and spores on ITO and GCE.
• folder "ITO_FL BF SECL" contains fluorescence (FL) and bright field (BF) and  SECL raw images of 1 micron beads, 450 nm silica beads,  200 nm and 100 nm PS beads recording on the ITO setup at 1.2 V vs Ag/AgCl. The solution is 0.1 M PBS (pH = 7.4) containing 1 mM [Ru(bpy)3]2+ and 100 mM TPA. 
• folder "particle stacking" contains data regarding:
 - FL and SECL images of tightly stacked cluster of 6 µm particles on glassy carbon electrode (GCE) recorded in the Procell solution contained 30 μM [Ru(bpy)3]2+ at 1.2 V. 
 - The decrease ratio calculated from the center, corner, edge and other single 6 µm particles. 
• folder "SECL FL intensity curve" contains data regarding:
- FL and SECL images of 6 µm, 3 µm and 200 nm particles on GCE and 100 nm particles on ITO. 
- The normalized FL and SECL intensity curve extracted from the FL and SECL images mentioned above. 
• folder "spores" contains data regarding:
 - the FL and the SECL images of spores on ITO and GCE.  
 - The normalized FL and SECL intensity curve extracted from the FL and SECL images of spores mentioned above.