Production of Biologically Synthesized Silver Nanoparticles using Glinus Oppositifolius Aqueous Leaf Extract; Characterization and Evaluation of its Anti-Bacterial Efficacy

The green synthesis of silver nanoparticles and their applications have attracted to applicable in many areas of research because of their therapeutic and clinical, and different applications, due to easily, safer, and lesser time and plant material. The synthesized nanoparticles achieved through greener way with the aqueous leaf source of Glinus oppositifolius. In the present work, bio-synthesized nanoparticles are characterized by advanced tools like UV-Vis Spectrophotometer, DLS-Zeta potential as well as the synthesized material was carried out the efficacy on two selected gram positive (Bacillus subtilis and Staphylococcus aureus) and two-gram negative (Klebsiella pneumonae and Escherichia coli) bacteria. The AgNPs formation was primarily confirmed through UV-Vis spectrophotometer scan ranges from 190 to 750 nm acquired intensive peak at 439 nm. The DLS particle size and zeta potential analysis revealed size and stability of the nanoparticle around 16.11 nm and – 31.4mV zeta potential values. The greener way synthesized silver nanoparticles of selected plant species AgNPs showed significant antibacterial activity on above mentioned bacteria. The DPPH activity expressed as significantly when compared with plant extract.