Published May 8, 2026
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A guide to selecting high-performing antibodies for Glucocorticoid receptor (UniProt ID: P04150) for use in western blot and flow cytometry
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1.
University of Leicester
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Description
Here we have characterized twenty-two NR3C1 commercial antibodies for use in western blot and flow cytometry using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. This work was supported by a grant from the National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) and MRC (NC3Rs Ref: NC/NAM0019/1, MRC UKRI076) alongside support from the Leicester Institute for Precision Health.