YCharOS - Antibody Characterization Through Open Science
Published May 8, 2026 | Version v1
Journal Open

A guide to selecting high-performing antibodies for Guanine nucleotide-binding protein G(q) subunit alpha (UniProt ID: P50148) for use in western blot and flow cytometry

Authors/Creators

  • 1. University of Leicester: Leicester, England, GB
  • 2. ROR icon University of Leicester

Contributors

Supervisor:

  • 1. The Montreal Neurological Institute

Description

Here we have characterized eleven GNAQ commercial antibodies for use in western blot and flow cytometry using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. This work was supported by a grant from the National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) and MRC (NC3Rs Ref: NC/NAM0019/1, MRC UKRI076) alongside support from the Leicester Institute for Precision Health.

Files

GNAQ YCharOS Report.pdf

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