Published July 13, 2026 | Version v2
Dataset Open

A broad-host-range Rhizobium rhizogenes strain enables transient expression across diverse crops and establishes functional assays in faba bean

Authors/Creators

  • 1. ROR icon Imperial College London
  • 2. ROR icon Academia Sinica
  • 3. ROR icon University of Cambridge
  • 4. ROR icon John Innes Centre
  • 5. ROR icon Sainsbury Laboratory

Description

Data structure:

Supplementary Data Fig 2. Betalain quantification for Fig 2B, corrected total cell fluorescence of GFP for Fig 2D, proportion of cells expressing GFP for Fig 2E, and GFP and mCherry fluorescence for Fig 2G.

Supplementary Data Fig 3. HR quantification at 800 nm emission for Fig 3B and 3D, and fluorescence intensity quantification for Fig 3E.

Supplementary Data Fig 4. HR quantification at 800 nm emission for Fig 4B and 4D.

Supplementary Data Fig 5. Haustoria counts and fluorescence intensity quantification for Fig 5C.

Supplementary Data Fig S1. Betalain quantification for Solanales species in Fig S1.

Supplementary Data Fig S2. Betalain quantification for N. tabacum cultivars in Fig S2.

Supplementary Data Fig S3. Betalain quantification for Lamiales and Gentianales species in Fig S3.

Supplementary Data Fig S4. Betalain quantification for Brassicales and Fabales species in Fig S4.

Supplementary Data Fig S5. Betalain quantification for Fig S5B, corrected total cell fluorescence of GFP for Fig S5D, and GFP fluorescence for Fig S5F.

Supplementary Data Fig S6. Amino acid alignment and maximum likelihood phylogeny underlying Fig S6.

Supplementary Data Fig S7. HR quantification for Fig S7A, S7B, S7C and S7D.

Raw gel images. Raw unedited images of agarose gels for RT-PCR depicted in Fig 2F and Fig S5G.

Scripts. All R scripts used for data visualisation and statistical testing.

Files

Raw gel images.zip

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Additional details

Related works

Is supplement to
Preprint: 10.64898/2026.04.20.719558 (DOI)