Saccharina latissima ONT BAM alignments for sequencing-based mutation density estimation (SeaMark Deliverable 1.7)

This dataset contains BAM alignment files generated from Oxford Nanopore sequencing of Saccharina latissima sporophytes derived from EMS‑mutagenized gametophytes.

The BAM files represent primary read alignments against the Saccharina latissima SLPER63f Female v2 reference genome and were produced using a standardized long‑read sequencing and alignment workflow. Reads were basecalled and demultiplexed using Dorado (super‑accurate model) and aligned using minimap2, with secondary alignments discarded.

These alignments were used for sequencing‑based confirmation of mutation load and for estimation of single‑nucleotide mutation density using a callable‑region and SNV‑based analysis framework. Callable genomic regions were defined based on minimum read‑depth criteria across samples, and downstream variant calling and filtering steps were applied to infer mutation density from the aligned reads.

The dataset is provided to support transparency, reproducibility, and reuse of alignment‑level data. It enables independent assessment of read alignment quality, coverage properties, and alternative downstream analyses. No processed variant call files, summary statistics, or interpretative results are included.