Published April 29, 2026
| Version v1
Journal article
Open
Discovery of two new Cystobasidiaceae species (Cystobasidiales, Cystobasidiomycetes) on phylloplane from western China
Authors/Creators
- 1. School of Life Science, Nanyang Normal University, Nanyang, China
- 2. School of Life Science, Nanyang Normal University, Nanyang, China|Research Center of Henan Provincial Agricultural Biomass Resource Engineering and Technology, Nanyang Normal University, Nanyang, China
Description
The fungal family Cystobasidiaceae, which includes the genera Begerowomyces, Cystobasidium, Cystastrum, Halobasidium, Robertozyma, and Queiroziella, comprises species that are predominantly associated with plants in tropical to cold regions. However, members of the genera Queiroziella and Robertozyma remain poorly studied. In the present study, fungal species were identified using a polyphasic approach combining phenotypic characterization and molecular phylogenetic analyses based on the D1/D2 domain of the large subunit (LSU) rRNA gene, the internal transcribed spacer (ITS) region, and the second largest subunit of RNA polymerase II (RPB2) gene. As a result, two novel species, Queiroziella pini sp. nov. (holotype CICC 33638T) and Robertozyma xinjiangensis sp. nov. (holotype GDMCC 2.525T), are proposed and described herein. Detailed descriptions, illustrations, and phylogenetic analyses of the novel taxa are provided, and comparisons with closely related species are presented. This study expands the known diversity of the family Cystobasidiaceae in China and provides a basis for future taxonomic and ecological investigations on this family.
Files
MC_article_185583.pdf
Files
(2.1 MB)
| Name | Size | Download all |
|---|---|---|
|
md5:c27bb613480a46222f98273989425125
|
1.9 MB | Preview Download |
|
md5:865487308ea7c01d78ce18d317a2c61c
|
174.2 kB | Preview Download |
Linked records
Additional details
References
- Bai FY, Takashima M, Hamamoto M, Nakase T (2001) Sporobolomyces yunnanensis sp. nov., a Q-10(H2)-containing yeast species with a close phylogenetic relationship to Erythrobasidium hasegawianum. International Journal of Systematic and Evolutionary Microbiology 51(1): 231–235. https://doi.org/10.1099/00207713-51-1-231
- Bauer R, Begerow D, Sampaio JP, Weiß M, Oberwinkler F (2006) The simple-septate basidiomycetes: a synopsis. Mycological Progress 5: 41–66. https://doi.org/10.1007/s11557-006-0502-0
- Chai CY, Lei T, Chu XY, Hui FL (2023) Multi-gene phylogeny and taxonomy of the genus Bannoa with the addition of three new species from central China. Frontiers in Microbiology 14: 1143156. https://doi.org/10.3389/fmicb.2023.1143156
- Chang CF, Lee CF, Liu SM (2019) Cystobasidium keelungensis sp. nov., a novel mycosporine producing carotenogenic yeast isolated from the sea surface microlayer in Taiwan. Archives Of Microbiology 201(1): 27–33. https://doi.org/10.1007/s00203-018-1570-7
- Crous PW, Wingfield MJ, Burgess TI, Hardy GESJ, Gené J, Guarro J, Baseia IG, García D, Gusmão LFP, Souza-Motta CM, Thangavel R, Adamčík S, Barili A, Barnes CW, Bezerra JDP, Bordallo JJ, Cano-Lira JF, de Oliveira RJV, Ercole E, Hubka V, Iturrieta-González I, Kubátová A, Martín MP, Moreau PA, Morte A, Ordoñez ME, Rodríguez A, Stchigel AM, Vizzini A, Abdollahzadeh J, Abreu VP, Adamčíková K, Albuquerque GMR, Alexandrova AV, Álvarez Duarte E, Armstrong-Cho C, Banniza S, Barbosa RN, Bellanger JM, Bezerra JL, Cabral TS, Caboň M, Caicedo E, Cantillo T, Carnegie AJ, Carmo LT, Castañeda-Ruiz RF, Clement CR, Čmoková A, Conceição LB, Cruz RHSF, Damm U, da Silva BDB, da Silva GA, da Silva RMF, de A Santiago ALCM, de Oliveira LF, de Souza CAF, Déniel F, Dima B, Dong G, Edwards J, Félix CR, Fournier J, Gibertoni TB, Hosaka K, Iturriaga T, Jadan M, Jany JL, Jurjević Ž, Kolařík M, Kušan I, Landell MF, Leite Cordeiro TR, Lima DX, Loizides M, Luo S, Machado AR, Madrid H, Magalhães OMC, Marinho P, Matočec N, Mešić A, Miller AN, Morozova OV, Neves RP, Nonaka K, Nováková A, Oberlies NH, Oliveira-Filho JRC, Oliveira TGL, Papp V, Pereira OL, Perrone G, Peterson SW, Pham THG, Raja HA, Raudabaugh DB, Řehulka J, Rodríguez-Andrade E, Saba M, Schauflerová A, Shivas RG, Simonini G, Siqueira JPZ, Sousa JO, Stajsic V, Svetasheva T, Tan YP, Tkalčec Z, Ullah S, Valente P, Valenzuela-Lopez N, Abrinbana M, Viana Marques DA, Wong PTW, Xavier de Lima V, Groenewald JZ (2018) Fungal Planet description sheets: 716–784. Persoonia 40: 240–393. https://doi.org/10.3767/persoonia.2018.40.10
- do Carmo-Sousa L, Phaff HJ (1962) An improved method for the detection of spore discharge in the Sporobolomycetaceae. Journal of Bacteriology 83(2): 434–435. https://doi.org/10.1128/jb.83.2.434-435.1962
- Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4): 783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
- Fell JW, Boekhout T, Fonseca A, Scorzetti G, Statzell-Tallman A (2000) Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. International Journal of Systematic and Evolutionary Microbiology 50(3): 1351–1371. https://doi.org/10.1099/00207713-50-3-1351
- Fotedar R, Fell JW, Boekhout T, Kolecka A, Zeyara A, Kaul R, Malki AA, Marri MA (2019) Cystobasidium halotolerans sp. nov., a novel basidiomycetous yeast species isolated from the Arabian Gulf. International Journal of Systematic and Evolutionary Microbiology 69(3): 839–845. https://doi.org/10.1099/ijsem.0.003250
- Gomes FC, Safar SV, Marques AR, Medeiros AO, Santos AR, Carvalho C, Lachance MA, Sampaio JP, Rosa CA (2015) The diversity and extracellular enzymatic activities of yeasts isolated from water tanks of Vriesea minarum, an endangered bromeliad species in Brazil, and the description of Occultifur brasiliensis f.a., sp. nov. Antonie Van Leeuwenhoek 107(2): 597–611. https://doi.org/10.1007/s10482-014-0356-4
- Guo Z, Wang Y, Hou Q, Li W, Zhao H, Sun Z, Zhang Z (2019) Halobasidium xiangyangense gen. nov., sp. nov., a new xylose-utilizing yeast in the family Cystobasidiaceae, isolated from the pickling sauce used to make Datoucai, a high-salt fermented food. International Journal of Systematic and Evolutionary Microbiology 69(1): 39–145. https://doi.org/10.1099/ijsem.0.003119
- Guo Y, Alshahni MM, Satoh K, Tamura T, Alshahni RZ, Makimura K (2023) Begerowomyces aurantius sp. nov., a novel yeast isolated from koalas' habitat in a Japanese zoological park. Journal of Veterinary Medical Science 85(3): 271–278. https://doi.org/10.1292/jvms.22-0374
- Hall TA (1999) Bioedit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
- Hamamoto M, Thanh VN, Nakase T (2002) Bannoa hahajimensis gen. nov., sp. nov., and three related anamorphs, Sporobolomyces bischofiae sp. nov., Sporobolomyces ogasawarensis sp. nov. and Sporobolomyces syzygii sp. nov., yeasts isolated from plants in Japan. International Journal of Systematic and Evolutionary Microbiology 52: 1023–1032. https://doi.org/10.1099/00207713-52-3-1023
- Hwang H, Bai J, Sathiyaraj S (2025) Characterization of two unrecorded yeast species in the families Cystobasidiaceae and Sporidiobolaceae isolated from Craspedonotus tibialis and mountain soil in Korea. Journal of Species Research 14: 74–80.
- Iurkov AM, Vustin MM, Tiaglov BV, Maksimova IA, Sineokiĭ SP (2008) Pigmented basidiomycete yeasts are a promising source of carotenoids and ubiquinone Q10. Mikrobiologiia 77(1): 5–10. https://doi.org/10.1134/S0026261708010013
- Jiang YL, Bao WJ, Liu F, Wang GS, Yurkov AM, Ma Q, Hu ZD, Chen XH, Zhao WN, Li AH, Wang QM (2024) Proposal of one new family, seven new genera and seventy new basidiomycetous yeast species mostly isolated from Tibet and Yunnan provinces, China. Studies in Mycology 109: 57–153. https://doi.org/10.3114/sim.2024.109.02
- Kachalkin A, Tomashevskaya M, Pankratov T, Yurkovet A (2024) Endothallic yeasts in the terricolous lichens Cladonia. Mycological Progress 23: 29. https://doi.org/10.1007/s11557-024-01966-0
- Katoh K, Rozewicki J, Yamada KD (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization, Briefings in Bioinformatics 20: 1160–1166. https://doi.org/10.1093/bib/bbx108
- Khunnamwong P, Surussawadee J, Jindamorakot S, Ribeiro JRA, Hagler AN, Limtong S (2015) Occultifur tropicalis f.a., sp. nov., a novel cystobasidiomycetous yeast species isolated from tropical regions. International Journal of Systematic and Evolutionary Microbiology 65(5): 1578–1582. https://doi.org/10.1099/ijs.0.000140
- Khunnamwong P, Ribeiro JRA, Garcia KM, Hagler AN, Takashima M, Ohkuma M, Endoh R, Sugita T, Jindamorakot S, Limtong S (2017) Occultifur plantarum f.a., sp. nov., a novel cystobasidiomycetous yeast species. International Journal of Systematic and Evolutionary Microbiology (8): 2628–2633. https://doi.org/10.1099/ijsem.0.001988
- Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33: 1870–1874. https://doi.org/10.1093/molbev/msw054
- Kurtzman CP, Robnett CJ (1998) Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73(4): 331–371. https://doi.org/10.1023/A:1001761008817
- Kurtzman CP, Robnett CJ (2015) Occultifur kilbournensis f.a. sp. nov., a new member of the Cystobasidiales associated with maize (Zea mays) cultivation. Antonie Van Leeuwenhoek 107(5): 1323–1329. https://doi.org/10.1007/s10482-015-0427-1
- Kurtzman CP, Fell JW, Boekhout T (2011) Methods for isolation, phenotypic characterization and maintenance of yeasts. In: Kurtzman CP, Fell JW, Boekhout T (Eds) The Yeasts – a Taxonomic Study, 5th edn, vol. 1. Amsterdam, Elsevier, 87–110. https://doi.org/10.1016/B978-0-444-52149-1.00007-0
- Laich F, Vaca I, Chávez R (2013) Rhodotorula portillonensis sp. nov., a basidiomycetous yeast isolated from Antarctic shallow-water marine sediment. International Journal of Systematic and Evolutionary Microbiology 63(10): 3884–3891. https://doi.org/10.1099/ijs.0.052753-0
- Li AH, Yuan FX, Groenewald M, Bensch K, Yurkov AM, Li K, Han PJ, Guo LD, Aime MC, Sampaio JP, Jindamorakot S, Turchetti B, Inacio J, Fungsin B, Wang QM, Bai FY (2020) Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species. Studies in Mycology 96: 17–140.
- Matheny PB, Gossman JA, Zalar P, Arun Kumar TK, Hibbett DS (2006) Resolving the phylogenetic position of the Wallemiomycetes: an enigmatic major lineage of Basidiomycota. Canadian Journal of Botany 84: 1794–1805. https://doi.org/10.1139/b06-128
- Nagahama T, Hamamoto M, Nakase T, Horikoshi K (2003) Rhodotorula benthica sp. nov. and Rhodotorula calyptogenae sp. nov., novel yeast species from animals collected from the deep-sea floor, and Rhodotorula lysiniphila sp. nov., which is related phylogenetically. International Journal of Systematic and Evolutionary Microbiology 53(3): 897–903. https://doi.org/10.1099/ijs.0.02395-0
- Parra PP (2019) Taxonomic studies and evolution of habitat preference in the Cystobasidiomycetes. Dissertation, Purdue University, USA.
- Sampaio JP, Bauer R, Begerow D, Oberwinkler F (1999) Occultifur externus sp. nov., a new species of simple-septate auricularioid heterobasidiomycete from plant litter in Portugal. Mycologia 91(6): 1094–1101. https://doi.org/10.1080/00275514.1999.12061118
- Schoutteten N, Yurkov A, Spirin V, Savchenko A, Aime MC, Begerow D, Verbeken A (2024) Examination of mycoparasites reveals a new type of host-parasite interface and rearranges the taxonomy of Occultifur and Microsporomyces (Cystobasidiomycetes, Basidiomycota). Studies in Mycology 109: 451–486. https://doi.org/10.3114/sim.2024.109.07
- Scorzetti G, Fell JW, Fonseca A, Statzell-Tallman A (2002) Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Research 2(4): 495–517. https://doi.org/10.1111/j.1567-1364.2002.tb00117.x
- Šibanc N, Zalar P, Schroers HJ, Zajc J, Pontes A, Sampaio JP, Maček I (2018) Occultifur mephitis f.a., sp. nov. and other yeast species from hypoxic and elevated CO2 mofette environments. International Journal of Systematic and Evolutionary Microbiology 68(7): 2285–2298. https://doi.org/10.1099/ijsem.0.002824
- Tanimura A, Sugita T, Endoh R, Ohkuma M, Kishino S, Ogawa J, Shima J, Takashima M (2018) Lipid production via simultaneous conversion of glucose and xylose by a novel yeast, Cystobasidium iriomotense. PLoS ONE 13(9): e0202164. https://doi.org/10.1371/journal.pone.0202164
- Tsuji M, Tsujimoto M, Imura S (2016) Cystobasidium tubakii and Cystobasidium ongulense, new basidiomycetous yeast species isolated from East Ongul Island, East Antarctica. Mycoscience 58(2): 103–110. https://doi.org/10.1016/j.myc.2016.11.002
- Turchetti B, Selbmann L, Gunde-Cimerman N, Buzzini P, Sampaio JP, Zalar P (2018) Cystobasidium alpinum sp. nov. and Rhodosporidiobolus oreadorum sp. nov. from European Cold Environments and Arctic Region. Life (Basel) 8(2): 9. https://doi.org/10.3390/life8020009
- Wang QM, Theelen B, Groenewald M, Bai FY, Boekhout T (2014) Moniliellomycetes and Malasseziomycetes, two new classes in Ustilaginomycotina. Persoonia 33: 41–47. https://doi.org/10.3767/003158514X682313
- Wang QM, Yurkov AM, Göker M, Lumbsch HT, Leavitt SD, Groenewald M, Theelen B, Liu XZ, Boekhout T, Bai FY (2015) Phylogenetic classification of yeasts and related taxa within Pucciniomycotina. Studies in Mycology 81: 149–189. https://doi.org/10.1016/j.simyco.2015.12.002
- White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (Eds) PCR Protocols: A Guide to Methods and Applications. Academic Press, New York, 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
- Yurkov AM, Vustin MM, Tyaglov BV, Maksimova IA, Sineokiy SP (2008) Pigmented basidiomycetous yeasts are a promising source of carotenoids and ubiquinone Q10. Microbiology 77: 1–6. https://doi.org/10.1134/S0026261708010013
- Yurkov AM, Kachalkin AV, Daniel HM, Groenewald M, Libkind D, de Garcia V, Zalar P, Gouliamova DE, Boekhout T, Begerow D (2015) Two yeast species Cystobasidium psychroaquaticum f.a. sp. nov. and Cystobasidium rietchieii f.a. sp. nov. isolated from natural environments, and the transfer of Rhodotorula minuta clade members to the genus Cystobasidium. Antonie Van Leeuwenhoek 107(1): 173–185. https://doi.org/10.1007/s10482-014-0315-0
- Zhang D, Gao F, Jakovlić I, Zou H, Zhang J, Li WX, Wang GT (2020) PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Molecular Ecology Resources 20(1): 348–355. https://doi.org/10.1111/1755-0998.13096
- Zhao JH, Bai FY, Guo LD, Jia JH (2002) Rhodotorula pinicola sp. nov., a basidiomycetous yeast species isolated from xylem of pine twigs. FEMS Yeast Research 2(2): 159–163. https://doi.org/10.1111/j.1567-1364.2002.tb00080.x