Temporal and Spatial Signatures of Synaptic Pathology in Cortical α-Synucleinopathy

Tabular data for the following experiments:

1 Analysis of synaptic pSYN localization, cortical mesoscale and layer-wise synaptic density_AS: Synaptic localization of pSYN and synaptic density  were quantified. Basically, it has the following dataset:

• Large-area quantification of how synaptically associated p-a-syn pathology impacts synaptic abundance
• SEQUIN analysis for 1MPI using synapsin and PSD-95 at layer 2/3 and layer 5
• Correlated synaptic pathology and excitatory synapse loss across connected cortical layers

SEQUIN for VGLUT1, VGLUT2, and VGAT-positive synapses_SS: Synapse densities for VGLUT1- and VGLUT2-positive excitatory synaptic loci and VGAT-positive inhibitory synaptic loci were quantified using SEQUIN. The separation changes between pre-VGLUT1- and VGLUT2-positive excitatory synaptic loci and post-Homer were determined using SEQUIN. 

VGLUT1 and VGLUT2 colocalization with endogenous α-synuclein_SS: Colocalization of VGLUT1 and VGLUT2 with endogenous α-synuclein was quantified in the cortex.

Electron Microscopy analysis of excitatory synapses_JR_DR: Ultrastructural measures were quantified, including synaptic vesicle area, intervesicular distance, and synaptic cleft distance.

pSyn pathology negatively impacts excitatory transmission_JG: Electrophysiology data from cortical slices were analyzed to assess how pSyn pathology affects synaptic transmission.

pSyn pathology progressively increases_SS: Lewy pathology progression at 1, 2, and 3 months post–striatal PFF injection was quantified.