Queiroziella pini C. Y. Chai & F. L. Hui sp. nov.

Fig. 3

Etymology.

The specific epithet pini refers to Pinus, the plant genus from which the type strain was isolated.

Typus.

China • Guizhou Province, Pingtang County, Sifangjing Village, on the phylloplane of Pinus sp., March 2024, D. Lu, NYNU 24343 (holotype CICC 33638 T preserved in a metabolically inactive state, metabolically inactive ex-type culture PYCC 10055).

Description.

On YM agar after 7 days at 20 ° C, the streak culture is pink to salmon, tough, smooth and glossy with an entire margin. In YM broth after 3 days at 20 ° C, cells are ellipsoidal (3.9–8.0 × 4.2–9.4 µm) and occur singly or in pairs. Budding is polar. After a month in YM broth at 20 ° C, a ring and a sediment are formed. In Dalmau plate culture on CMA at 20 ° C, pseudohyphae and hyphae are not formed. Sexual structures are not observed on PDA, CMA or V 8 agar at 17 ° C. Ballistoconidia are not produced. Glucose fermentation is absent. The following carbon sources are assimilated: glucose, inulin (weak and delayed), sucrose, raffinose, melibiose (delayed), D-galactose, trehalose, maltose, melezitose, cellobiose, salicin, D-xylose, L-arabinose (weak), D-arabinose, 5 - keto-D-gluconate, d-ribose, glycerol, erythritol, ribitol, galactitol, D-mannitol, D-glucitol, DL-lactate (weak), succinate, D-gluconate (delayed), N-acetyl-D-glucosamine, 2 - keto-d-gluconate, D-glucuronate (delayed), and d-glucono- 1, 5 - lactone. Lactose, methyl-α-D-glucoside, L-sorbose, L-rhamnose, methanol, ethanol, myo - inositol, citrate, and D-glucosamine are not assimilated. L-Lysine is assimilated as sole nitrogen sources. Nitrate, nitrite, ethylamine, and cadaverine are not assimilated. Growth is observed at 25 ° C, but not at 30 ° C. Growth on 50 % (w / w) glucose-yeast extract agar is negative. Urease activity and diazonium blue B reaction are positive.

Additional strain examined.

China • Guizhou Province, Pingtang County, Sifangjing Village, on the phylloplane of Ormosia henryi, March 2024, D. Lu, NYNU 24393.

GenBank accession numbers.

Holotype CICC 33638 T (ITS: PP 837677, LSU: PP 849682, RPB 2: PZ 233424); additional strain NYNU 24393 (ITS: PP 849684, LSU: PP 849683, RPB 2: PZ 233425).

Note.

Physiologically, Q. pini differs from the closely related species Q. brasiliensis in its ability to assimilate salicin D-xylose, L-arabinose, galactitol, and succinate and its inability to assimilate L-sorbose and grow at 30 ° C (Table 2).

* Data for reference species were taken from Crous et al. (2018) and Li et al. (2020). +, positive; –, negative; d, delayed positive; v, variable; w, weakly positive; n, not available.