Published March 19, 2025
| Version v1
Conference paper
Open
Large-area dynamic contrast MHz optical coherence tomography for label-free imaging of porcine tissue
Authors/Creators
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Burhan, Sazgar
(Researcher)1
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Göb, Madita
(Researcher)1
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Pieper, Mario
(Researcher)2
- Laedtke, Tjalfe (Researcher)1
- Grahl, Thorge (Researcher)1
- Münter, Michael (Researcher)1
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Schulz-Hildebrandt, Hinnerk
(Researcher)3, 4
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Hüttmann, Gereon
(Supervisor)1, 5
- König, Peter (Supervisor)1
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Huber, Robert
(Supervisor)6, 1
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1.
University of Lübeck
- 2. University of Luebeck
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3.
Massachusetts General Hospital
-
4.
Harvard Medical School
- 5. Medical Laser Center Lübeck GmbH
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6.
Medizinisches Laserzentrum Lübeck (Germany)
Description
We demonstrate a 3.2 MHz-OCT system for inter-volumetric dynamic optical coherence tomography of ex vivo porcine kidney tissue. Employing a home-built Fourier Domain mode locking (FDML) laser with a 1310 nm wavelength, the system achieved a lateral resolution of 3.48 μm and a frame rate of 612 Hz. A motorized XYZ positioning stage enabled the precise acquisition of multiple volumes, which were seamlessly stitched together to generate a comprehensive dataset with a total area of 2.6 × 2.6 mm2. Validations against histological sections confirmed the system’s ability to visualize cellular tissue structures.
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