Published April 24, 2026 | Version 3.25.0
Software Open

nf-core/rnaseq: nf-core/rnaseq v3.25.0 - Plutonium Pangolin

  • 1. Seqera
  • 2. @seqeralabs
  • 3. Boehringer Ingelheim
  • 4. @seanome
  • 5. @SciLifeLab
  • 6. Inivata
  • 7. Johns Hopkins University
  • 8. NVIDIA
  • 9. @NBISweden
  • 10. @qbicsoftware
  • 11. Seqera Labs
  • 12. Francis Crick Institute @crickbabs
  • 13. University of Pavia
  • 14. HKI Jena, MPI-EVA Leipzig
  • 15. @ScilifelabDataCentre
  • 16. >_ glich
  • 17. CRG

Description

What's Changed

  • Bump version to 3.25.0dev; fix SortMeRNA sample name in MultiQC by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1781
  • refactor: delocalise GTF_FILTER to nf-core custom/gtffilter by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1782
  • fix: wire up skip_gtf_transcript_filter to CUSTOM_GTFFILTER ext.args by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1783
  • Replace local gtf2bed with nf-core ea-utils/gtf2bed module by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1784
  • refactor: replace local BAM QC with nf-core bam_qc_rnaseq subworkflow by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1786
  • feat: add GPU support for ribodetector by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1790
  • refactor: centralize module configs in conf/modules/ by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1788
  • Bump custom/multiqccustombiotype to fail loudly on high biotype cardinality by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1795
  • feat: add strand-agnostic bigwig, skip per-strand for unstranded libraries by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1792
  • Fix MultiQC collapsing _1/_2 sample IDs via per-sample lookbehind by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1793
  • docs: clarify prokaryotic transcript extraction includes tRNA/rRNA/tmRNA/ncRNA by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1796
  • Add stringtie_merge for de novo assembly by @Odulhin in https://github.com/nf-core/rnaseq/pull/1755
  • fix(prokaryotic): skip StringTie by default in the prokaryotic profile by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1804
  • fix: per-sample MultiQC progressive closure via structural .join chain by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1803
  • feat(bowtie2_salmon): report up to -k 200 alignments so Salmon EM can work by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1806
  • fix(sortmerna): adopt SortMeRNA SILVA 138 smr_v4.3_default_db as the default rRNA database by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1811
  • fix(mqc): strandedness table rows reflect which analyses ran; narrow prokaryotic RSeQC skip by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1805
  • test: dedupe pipeline-level tests without losing coverage by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1812
  • chore: sync nf-core components and retire ch_versions plumbing by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1814
  • test(cleanup): gate pipeline-test cleanup on CI to keep local reruns stable by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1815
  • Bump version to 3.25.0 ahead of release by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1799
  • fix(prokaryotic): derive gene BED via gffread --bed by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1817
  • chore: drop redundant versions.yml filters from saveAs closures by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1818
  • fix(prokaryotic): skip RSeQC infer_experiment on bowtie2_salmon + skip sentieon on conda by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1819
  • Dev -> master for nf-core/rnaseq 3.25.0 by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1816

New Contributors

  • @Odulhin made their first contribution in https://github.com/nf-core/rnaseq/pull/1755

Full Changelog: https://github.com/nf-core/rnaseq/compare/3.24.0...3.25.0

Files

nf-core/rnaseq-3.25.0.zip

Files (7.2 MB)

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Additional details

Related works

Is supplement to
Software: https://github.com/nf-core/rnaseq/tree/3.25.0 (URL)

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