Morphological and molecular evidence support a new species of the genus Ilocomba Brescovit, 1997 (Araneae, Anyphaenidae, Anyphaeninae) from the Andes of Colombia
Authors/Creators
- 1. Grupo de Investigación Biodiversidad del Caribe, Departamento de Biología, Universidad del Atlántico, Barranquilla, Colombia|División Aracnología, Museo Argentino de Ciencias Naturales "Bernardino Rivadavia" CONICET, Buenos Aires, Argentina|Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina|Grupo de Estudios en Sistemática y Conservación, Universidad del Atlántico, Barranquilla, Colombia
- 2. Grupo de Investigación Biodiversidad del Caribe, Departamento de Biología, Universidad del Atlántico, Barranquilla, Colombia
- 3. Universidad Nacional de Colombia, Sede Bogotá, Facultad de Ciencias, Instituto de Ciencias Naturales, Bogotá, Colombia|Grupo de investigación Biología, Ecología y Evolución de Artrópodos, Departamento de Biología, Universidad del Valle, Cali, Colombia
Description
We describe a new species of the genus Ilocomba Brescovit, 1997 from the Andean region of Colombia using an integrative taxonomic approach that combines morphological and molecular data. We assess the phylogenetic position of the new species using four molecular markers: COI, 16S, 28S, and H3. The distinctiveness of the new species, Ilocomba yotoco sp. nov., is supported by its placement in the phylogenetic tree, unique morphological characteristics, and uncorrected pairwise genetic distances. We also provide new morphological data and geographic records for Ilocomba marta Brescovit, 1997. Additionally, we present a distribution map of the genus and an updated identification key for all known species of Ilocomba.
Files
ZK_article_162601.pdf
Files
(21.4 MB)
| Name | Size | Download all |
|---|---|---|
|
md5:cdfc82b2c395515431b9dccdac44c7ae
|
21.2 MB | Preview Download |
|
md5:4bc124c9690ff8d07dd2d987da62b7d1
|
202.3 kB | Preview Download |
Linked records
Additional details
References
- Abhijith APC, Hill DE, Ramachandra P (2022) Notes on biology of the ant-mimicking jumping spider Myrmarachne plataleoides (Araneae: Salticidae: Astioida) in south Asia. Peckhamia 287(1): 1–12.
- Álvarez-Padilla F, Hormiga G (2007) A protocol for digesting internal soft tissues and mounting spiders for scanning electron microscopy. The Journal of Arachnology 35(3): 538–542. https://doi.org/10.1636/Sh06-55.1
- Barone ML, Wilson JD, Zapata L, Soto EM, Haddad CR, Grismado C, Izquierdo M, Arias E, Pizarro-Araya J, Briones R, Barriga JE, Peralta L, Ramírez MJ (2024) Genetic barcodes for species identification and phylogenetic estimation in ghost spiders (Araneae: Anyphaenidae: Amaurobioidinae). Invertebrate Systematics 38(11): 1–18. https://doi.org/10.1071/IS24053
- Brescovit AD (1997) Revisão de Anyphaeninae Bertkau a nível de gêneros na região neotropical (Araneae, Anyphaenidae). Revista Brasileira de Zoologia 13(suppl 1): 1–187. https://doi.org/10.1590/S0101-81751996000500001
- Casquet J, Thebaud C, Gillespie RG (2012) Chelex without boiling, a rapid and easy technique to obtain stable amplifiable DNA from small amounts of ethanol‐stored spiders. Molecular ecology resources 12(1): 136–141. https://doi.org/10.1111/j.1755-0998.2011.03073.x
- Colgan DJ, McLauchlan A, Wilson GDF, Livingston SP, Edgecombe GD, Macaranas J, Cassis G, Gray MR (1998) Histone H3 and U2 snRNA sequences and arthropod molecular evolution. Australian Journal of Zoology 46(5): 419–437. https://doi.org/10.1071/ZO98048
- Dupérré N (2023) Araneae (spiders) of South America: A synopsis of current knowledge. New Zealand Journal of Zoology 50(1): 3–117. https://doi.org/10.1080/03014223.2021.2022722
- Ewing B, Green P (1998) Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Research 8(3): 186–194. https://doi.org/10.1101/gr.8.3.186
- Ewing B, Hillier L, Wendl MC, Green P (1998) Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Research 8(3): 175–185. https://doi.org/10.1101/gr.8.3.175
- Foellmer MW, Fairbairn DJ (2005) Sexual selection research on spiders: Progress and biases. Biological Reviews of the Cambridge Philosophical Society 80(3): 363–385. https://doi.org/10.1017/S1464793104006700
- Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3: 294–299.
- Funke S, Huber BA (2005) Allometry of genitalia and fighting structures in Linyphia triangularis (Araneae: Linyphiidae). The Journal of Arachnology 33(3): 870–872. https://doi.org/10.1636/S04-16.1
- Goloboff PA, Morales ME (2023) TNT version 1.6, with a graphical interface for MacOS and Linux, including new routines in parallel. Cladistics 39(2): 144–153. https://doi.org/10.1111/cla.12524
- Gordon D, Abajian C, Green P (1998) Consed: A graphical tool for sequence finishing. Genome Research 8(3): 195–202. https://doi.org/10.1101/gr.8.3.195
- Gordon D, Desmarais C, Green P (2001) Automated finishing with autofinish. Genome Research 11(4): 614–625. https://doi.org/10.1101/gr.171401
- Guindon S, Dufayard J-F, Lefort V, Anisimova M, Hordijk W, Gascuel O (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: Assessing the performance of PhyML 3.0. Systematic Biology 59(3): 307–321. https://doi.org/10.1093/sysbio/syq010
- Hoang DT, Chernomor O, von Haeseler A, Minh BQ, Vinh LS (2018) UFBoot2: Improving the ultrafast bootstrap approximation. Molecular Biology and Evolution 35(2): 518–522. https://doi.org/10.1093/molbev/msx281
- Kalyaanamoorthy S, Minh BQ, Wong TKF, von Haeseler A, Jermiin LS (2017) ModelFinder: Fast model selection for accurate phylogenetic estimates. Nature Methods 14(6): 587–589. https://doi.org/10.1038/nmeth.4285
- Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Molecular Biology and Evolution 30(4): 772–780. https://doi.org/10.1093/molbev/mst010
- Labarque FM, Soto EM, Ramírez MJ, Arnedo MA (2015) Chasing ghosts: The phylogeny of Amaurobioidinae ghost spiders (Araneae, Anyphaenidae). Zoologica Scripta 44(5): 550–561. https://doi.org/10.1111/zsc.12119
- Larsson A (2014) AliView: A fast and lightweight alignment viewer and editor for large datasets. Bioinformatics 30(22): 3276–3278. https://doi.org/10.1093/bioinformatics/btu531
- Martínez L, Brescovit AD, Martínez N (2018) Five new species of the Ghost Spider genus Anyphaenoides Berland from Colombia (Araneae: Anyphaenidae: Anyphaeninae). Zootaxa 4425(2): 357–371. https://doi.org/10.11646/zootaxa.4425.2.10
- Martínez L, Brescovit AD, Oliveira LFM (2020) Two new species of the ghost spider genus Macrophyes O. Pickard-Cambridge, 1893 from Colombia and description of the first male of Mesilla Simon, 1903 (Araneae: Anyphaenidae: Anyphaeninae). Zootaxa 4853(4): 581–590. https://doi.org/10.11646/zootaxa.4853.4.7
- Martínez L, Brescovit AD, Villarreal E, Oliveira LFM (2021) An update of morphological and distributional data of the genus Patrera Simon (Araneae: Anyphaenidae: Anyphaeninae) with the description of twenty-five new species from Colombia. Zootaxa 4914(1): 1–64. https://doi.org/10.11646/zootaxa.4914.1.1
- McLean CJ, Garwood RJ, Brassey CA (2018) Sexual dimorphism in the Arachnid orders. PeerJ 6: e5751. https://doi.org/10.7717/peerj.5751
- Nguyen L-T, Schmidt HA, von Haeseler A, Minh BQ (2015) IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution 32(1): 268–274. https://doi.org/10.1093/molbev/msu300
- Oliveira LFM (2023) Análise filogenética da subfamília Anyphaeninae Bertkau, 1878 (Araneae: Dionycha, Anyphaenidae). PhD Thesis, Universidade de São Paulo, São Paulo, Brazil.
- Oliveira LFM, Brescovit AD (2021) Taxonomic revision and cladistic analysis of ghost spiders of the genus Tafana Simon, 1903 (Araneae: Dionycha, Anyphaenidae), with the descriptions of twelve new species. European Journal of Taxonomy 742: 1–77. https://doi.org/10.5852/ejt.2021.742.1291
- Oliveira LFM, Brescovit AD (2025) On the Neotropical spider genus Hatitia Brescovit, 1997 (Araneae: Anyphaenidae: Anyphaeninae), with the description of five new species. European Journal of Taxonomy 997: 180–209. https://doi.org/10.5852/ejt.2025.997.2933
- Paradis E, Schliep K (2019) ape 5.0: An environment for modern phylogenetics and evolutionary analyses in R. Bioinformatics 35(3): 526–528. https://doi.org/10.1093/bioinformatics/bty633
- Petrunkevitch A (1925) Arachnida from Panama. Transactions of the Connecticut Academy of Arts and Sciences 27: 51–248.
- QGIS Development Team (2023) QGIS Geographic Information System. Open Source Geospatial Foundation Project. https://qgis.org [accessed 29 Sep 2025]
- Simon C, Frati F, Beckenbach A, Crespi B, Liu H, Flook P (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America 87(6): 651–701. https://doi.org/10.1093/aesa/87.6.651
- Solano-Brenes D, García-Hernández S, Machado G (2018) All the better to bite you with! Striking intrasexual differences in cheliceral size define two male morphs in an Amazonian arachnid. Biological Journal of the Linnean Society. Linnean Society of London 125(3): 521–534. https://doi.org/10.1093/biolinnean/bly120
- Wheeler WC (2012) Systematics: a Course of Lectures. Wiley-Blackwell, Oxford, 368 pp. https://doi.org/10.1002/9781118301081
- Wheeler WC, Coddington JA, Crowley LM, Dimitrov D, Goloboff PA, Griswold CE, Hormiga G, Prendini L, Ramírez MJ, Sierwald P, Almeida-Silva L, Alvarez-Padilla F, Arnedo MA, Benavides Silva LR, Benjamin SP, Bond JE, Grismado CJ, Hasan E, Hedin M, Izquierdo MA, Labarque FM, Ledford J, Lopardo L, Maddison WP, Miller JA, Piacentini LN, Platnick NI, Polotow D, Silva-Dávila D, Scharff N, Szűts T, Ubick D, Vink CJ, Wood HM, Zhang J (2017) The spider tree of life: Phylogeny of Araneae based on target-gene analyses from an extensive taxon sampling. Cladistics 33(6): 574–616. https://doi.org/10.1111/cla.12182
- Whiting MF, Carpenter JC, Wheeler QD, Wheeler WC (1997) The Strepsiptera problem: Phylogeny of the holometabolous insect orders inferred from 18S and 28S ribosomal DNA sequences and morphology. Systematic Biology 46(1): 1–68. https://doi.org/10.1093/sysbio/46.1.1
- World Spider Catalog (2025) World Spider Catalog. Version 26.0. Natural History Museum Bern. https://doi.org/10.24436/2