Data for manuscript entitled " Metabolism fine tuning and cardiokines secretion represent adaptative responses of the heart to High Fat and High Sugar Diets in flies"

This repository contains Data and Raw images used for the analysis and the graphical representation used in the 6 figures and 6 supplemental figures of our manuscript. Each file contains compressed data corresponding to one figure and its associated supplemental figure.

• Figure 1_Figure S1: scaled data (.csv) of cardiac imaging
  • controlKK_60100
  • controlGD_60000
  • controlGFP

• Figure 2_Figure S2:
  • rnaseq report
  • genes lists for heatmap Figure 2E,F
  • r script for heatmap graphical representation

• Figure 3_Figure S3: contains 5 folders corresponding to each individual candidate gene validated and presented (Gnmt, Sardh, Galk, Snmp& and nebu).
  • For Gnmt, Snmp1, GalK and nebu: sub-folders
    • •  cardiac imaging: scaled data (.csv) of cardiac imaging
      • images: M-modes and positions lines, captured images
  • For Sardh: scaled data (.csv) of cardiac imaging
• Figure 4_Figure S4: scaled data (.csv) of cardiac imaging

• Figure 5_Figure S5: contains 4 sub-folders corresponding to genotypes presented in the figures' panels
  • HandfitSP, HandfitKK, HandfitWT: scaled data (.csv) of cardiac imaging
  • fit81: scaled data (.csv) of cardiac imaging and captured images

• RTqPCR Figures 3 and 5 hearts: data results and analysis (.xls)

• Figure 6_Figure S6: contains 4 sub-folders
  • • • Food intake: data raw and normalized (.xls)
      • Western blotting: raw images
      • Imaging confocal: 
        • • HandfitWT_HandfitKK_ starvation refeeding: raw images and projections, data analysis
          • HandfitOE ND HSD HFD: raw images and projections, data analysis
          • Hand FitKK ND: raw images and projections, data analysis