Method Development and Simultaneous Estimation of Amikacin Sulphate by Using UV and HPLC/ RP-HPLC

Severe Gram-negative bacterial infections are frequently treated with amikacin sulphate, a semi-synthetic aminoglycoside antibiotic. The development of trustworthy and precise analytical techniques is crucial for guaranteeing its purity, safety, and efficacy in pharmaceutical formulations because of its therapeutic significance. However, amikacin absence of a strong chromophore makes direct detection using traditional UV spectrophotometry extremely difficult. The method development and simultaneous measurement of amikacin sulphate utilising UV spectrophotometric and Reverse Phase High Performance Liquid Chromatography (RP-HPLC) methods are the main topics of this study. The method development and simultaneous measurement of amikacin sulphate utilising UV spectrophotometric and Reverse Phase High Performance Liquid Chromatography (RP-HPLC) methods are the main topics of this study. To improve UV detectability, a number of derivatization techniques have been explored, including the use of chemicals like ninhydrin and o-phthalaldehyde. RP-HPLC techniques are more suited for complex pharmaceutical formulations and combination medication analysis because of their increased sensitivity, specificity, and repeatability. Important aspects of method development are emphasised, including the choice of mobile phase, column type, pH, and detection wavelength. Additionally, a thorough assessment of the validation of analytical techniques in accordance with ICH criteria has been conducted, including factors such as linearity, accuracy, precision, robustness, and limits of detection and quantification. Additionally, a comparison of RP-HPLC and UV techniques is provided. In addition to analysing future prospects combining sophisticated analytical tools and green chemistry methods, the study highlights the significance of these analytical techniques in quality assurance, stability studies, and regulatory compliance.