A Comparative Study of Ultrafast Papanicolaou Staining with Standard Papanicolaou Staining Technique for the Assessment of Cervical Smear – A Hospital Based Observational Study

This prospective study was conducted to assess the use of ultrafast Papanicolaou stain for cervical smears.Study included 60 cervical smear cases. In each case, two smears were prepared and stained by UFp and standard Papanicolaou stains. The four parameters background, cell morphology,nuclear details and overall staining has been considered Quality index was calculated from ratio of score achieved to maximum possible score. Comparison is done between the two staining techniques on the basis of these cytomorphological features. It was observed that UFP-stained smears had clear RBC free background, crisp nuclear chromatin, well-stained nucleoli, and transparent cytoplasm. Hence, it was concluded that UFP stain is useful for rapid diagnosis for cervical smear which is time saving and helpful.