Cultures of healthy and ALS iPSCs-derived astrocytes immunolabeled for DAPI and GFAP

Data generation

Individual astrocyte cultures were generated across four distinct experimental batches (i.e., different inductions and culturing times) and immunolabeled using two fluorescent markers: (i) DAPI for nuclear staining and (ii) GFAP for intermediate filament visualization.
For each well, non-overlapping fields of view (FOVs; 1080×1080 pixels, 0.316 μm/px) were acquired at random locations. Upon processing, each image was subdivided into sixteen non-overlapping crops (270×270 pixels)

Raw image data

The archive (fluoACs_raw.zip) contains 6 folders corresponding to distinct 6 well plates from 4 experiments. The content of the archive is annotated in annotations_raw_wells.csv.
Each row corresponds to one well in the 96-well plates. Each well in the plate has 10 to 12 fields of view, each field of view has 4 to 5 planes and each plane was stained with 2 channels.
Each image is encoded as follows: 00R00C-F-00100P00H, where R is the row of the well in the plate, C is the column of the well in the plate, F is the field of view, P is the plane and H is the channel.

Processed images

The archive (fluoACs_processed.zip) contains processed RGB images (maximum intensity projection, contrast enhancement, channels merging, cropping). The content of the archive is annotated in annotations_processed_crops.csv, where each row corresponds to one crop.
Each image is encoded as follows: EX-PX-RWX-CWX-FX-CRX_16.tif, where X represents integer values for: E (Experiment), P (Plate), RW (Row), CW (Column), F (Field of View), and CR (Crop index). The suffix _16 denotes the crop is 1/16 of the original image.

Code using this data for identifying and analysing astrocyte phenotypic sub-states can be accessed on GitHub.
This data has been used in a manuscript posted on BioRxiv.