Genome editing-based refinement of GPCR visualization in mice using the oxytocin receptor as a model

This dataset contains all supplementary materials associated with the manuscript:
“Genome editing-based refinement of GPCR visualization in mice using the oxytocin receptor as a model” (Inoue YU et al., under review at Scientific Reports).

The files include raw and processed data used in the figures and analyses reported in the paper, as well as step-by-step experimental protocols to facilitate reproducibility.

Contents:

• In Cellulo GEF Assay Protocol.docx: Step-by-step protocol of in cellulo GEF assay.

• Raw Data_Fig. S7A-B.xlsx: Raw data used for Figures S7A and S7B.

• Raw Data_Fig. 2H-J & S7C-F.xlsx: Raw data used for Figures 2H-J and S7C-F.

• Raw Data_Fig. S9D.xlsx: Raw data used for Figure S9D.

• Raw Data_Fig. S9E.xlsx: Raw data used for Figure S9E.

• Raw Data_Fig. S9F.xlsx: Raw data used for Figure S9F.

• Input protein sequences for AlphaFold3 prediction.txt: Amino acid sequences used for AlphaFold3 structure prediction.

• Oxtr-3xHA.pse: Structural models of Oxtr-3xHA predicted by AlphaFold3.

• Oxtr-3xALFA.pse: Structural models of Oxtr-3xALFA predicted by AlphaFold3.

• Oxtr-smFP.pse: Structural models of Oxtr-smFP predicted by AlphaFold3.

• smFP.pse: Structural models of smFP predicted by AlphaFold3.

• Dataset_Fig. 2, S7 & S9.xlsx: Processed datasets used for Figures 2, S7, and S9.

All data were generated in the laboratory of Ikuo Masuho (Sanford Research).

Correspondence: ikuo.masuho@sanfordresearch.org

The dataset is made available under the CC-BY 4.0 license to promote transparency and reuse.