Published April 9, 2026
| Version v2
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Feasibility Study: Soluble Protein Expression Measured by HiBiT in E. coli and P. pastoris
Authors/Creators
- 1. The Align Foundation
- 2. C16 Biosciences
-
3.
Ginkgo Bioworks, Inc. (United States)
Contributors
Hosting institution:
Description
In this work, we aimed to test the feasibility of using the HiBiT assay for assessing protein expression in two microbial hosts: Escherichia coli and Pichia pastoris. These hosts represent distinct expression systems, bacterial and yeast, that serve as an ideal starting point to evaluate cross-organismal assay performance. The goal of this study is to determine whether a workflow involving a HiBiT-tagged approach to measuring soluble protein can reliably quantify expression levels across these systems and provide the necessary scalability and robustness required for broader implementation.
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Feasibility Study_UPDATE.pdf
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Additional details
Related works
- Continues
- Publication: 10.5281/zenodo.14014029 (DOI)