In vitro techniques in Solanum lycopersicum, Solanum tuberosum, and Solanum × muricatum anthers

In vitro techniques for Solanum transcendental crops were developed, including propagation from tomato seed, micropropagation of potato by tuber sprouts, and sweet pepino anther culture. At all stages of tomato, and potato a completely randomized design (CRD) with or without factorial arrangement was used; and culture media were assessed for sweet pepino anthers. Tomatoes Floradade (V1), and Montego (V2), potatoes Chaucha (CH), Leona Blanca (LB), and Leona Negra (LN), and sweet pepino New Generation (NG) were utilized, as well as two disinfection treatments (TD), different concentrations of gentamicin (50, 68.8, and 75 mg · L-1), fungicides (FF), bacteriostatic
and bactericidal (BB). Tomato germination with contamination < 10% was obtained with V1-TD1-68.8ppm = 86.67%, and V2-TD1-68.8ppm = 80.00%; while explant length (mean ± S.E.) ranging from V2-TD1-68.8ppm = 12.63 cm ± 0.49, to V2-TD2-68.8ppm = 3.40 cm ± 1.23 was significantly different; and ex vitro plantlet length was different between V1 = 12.64 cm ± 0.68, and V2 = 17.17 cm ± 0.69. In the micropropagation of potato (contamination 0.0-6.67% and survival 93.33-100%), leaf number was different among CH-FFBB-75ppm = 7.60 ± 0.43 (A), LB-FFBB-75ppm = 4.00 ± 0.58 (B), and LN-FFBB-75ppm = 1.50 ± 0.27 (C), and explant length was similar, with 2.14, 1.79, and 1.96 cm, respectively. Two calluses from sweet pepino anthers were obtained on media with kinetin (0.01-0.1 ppm), auxin, and cytokinin.