Published January 1, 2013 | Version v1
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Molecular characterization of foot and mouth disease viruses collected from Suez Canal area, Egypt from 2009 to 2011

Authors/Creators

  • 1. Virology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egyp
  • 2. Department of FMD, Veterinary Serum and Vaccine Research Institute (VSVRI), Abassia, Cairo, Egypt

Description

Foot and mouth disease virus (FMDV) is characterized by high genetic and antigenic variations. The nucleotide sequence of VP1 gene of FMDV serotypes O and A were determined and compared to the sequences of some national and international FMDV from GenBank. Six PCR positive samples were selected for sequencing as one from serotype O and A from the 3 governorates of Suez Canal area (Ismailia, Suez and Port Said). Results of the sequencing of VP1 gene of FMDV serotype A revealed that there are slight divergence (0.09% to 2.34%) among the isolated strains. While there is an obvious divergence (more than 13.77%) to the other compared FMDV strains (A/Egypt 2006, A/KEN/98, A/ETH/2007 and A/SUD/82). Also the results revealed that our isolates (FMD A/Ismailia, FMD A/ Suez and FMD A/Port Said) are closely related to FMD A/Egypt 2006. The results of the sequencing of VP1 gene (1D) of FMDV serotype O showed high similarity (0.32% to 0.97%) among the isolated strains. While there were a noticeable divergence (more than 10.35%) between these isolates and the other compared FMDV strains (O/Pan Asia, O/EGY/93, O/Manisa/87 and O/Campos/2000). Also the results revealed that our isolates (FMD O/Ismailia, FMD O/ Suez and FMD O/Port Said) are closely related to FMD O/Pan Asia isolates.

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Is supplement to
10.5281/zenodo.19335 (DOI)

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