Published March 26, 2026
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Multi-gene phylogeny and morphological evidence reveal two novel species and two new records of Acrogenospora (Minutisphaerales, Acrogenosporaceae) from aquatic habitats in mainland China
Authors/Creators
- 1. Jiangxi Key Laboratory for Excavation and Utilization of Agricultural Microorganisms, Jiangxi Agricultural University, Nanchang, China|Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang, China
- 2. Jiangxi Key Laboratory for Excavation and Utilization of Agricultural Microorganisms, Jiangxi Agricultural University, Nanchang, China|College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, China
- 3. Nanchang Normal University, Nanchang, China
- 4. Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang, China
- 5. Jiangxi Key Laboratory for Excavation and Utilization of Agricultural Microorganisms, Jiangxi Agricultural University, Nanchang, China
Description
Freshwater fungi are characterized by their high species diversity and perform vital ecological roles. During our survey of Chinese aquatic microfungal diversity, four fungal strains were isolated from freshwater environments in Jiangxi and Guizhou Provinces, China. Through comprehensive morphological assessment and multi-locus phylogenetic analyses based on combined ITS, LSU, SSU, RPB2, and TEF1-α sequences, two new species, Acrogenospora bambusae and A. lignicola, were introduced. In addition, two previously described species, A. taiwanica and A. thailandica, originally described from China (Taiwan), and Thailand, respectively, are newly reported from mainland China. This study provides detailed morphological descriptions, photographic illustrations, and phylogenetic placements for each taxonomic novelty.
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References
- Bao DF, McKenzie EHC, Bhat DJ, Hyde KD, Luo ZL, Shen HW, Su HY (2020) Acrogenospora (Acrogenosporaceae, Minutisphaerales) appears to be a very diverse genus. Frontiers in Microbiology 11: e1606. https://doi.org/10.3389/fmicb.2020.01606
- Boehm EW, Mugambi GK, Miller AN, Huhndorf SM, Marincowitz S, Spatafora JW, Schoch CL (2009) A molecular phylogenetic reappraisal of the Hysteriaceae, Mytilinidiaceae and Gloniaceae (Pleosporomycetidae, Dothideomycetes) with keys to world species. Studies in Mycology 64: 49–83. https://doi.org/10.3114/sim.2009.64.03
- Cao B, Phurbu D, Ralaiveloarisoa A, Liimatainen K, Niskanen T, Ramírez-Cruz V, Bradshaw AJ, Dentinger BTM, Ramírez-Guillén F, Cortés-Pérez A, Guzmán-Dávalos L, Villalobos-Arámbula AR, Castro-Jauregui O, Santos da Silva P, Day R, Davoodian N, Lebel T, Castellano M, May TW, Toome M, Vasey J, Hofer K, Thangavel R, Braithwaite M, Braithwaite L, Dutta AK, Chattopadhyay P, Roy N, Tanti B, Biswas PR, Arumugam E, Kezo K, Kaliyaperumal M, Murugadoss R, Ji JX, Kakishima M, Cooper J, Nuytinck J, Luo ZL, Wang WP, Zhang X, Wen TC, Jia AL, Fan XL, Jiang N, Jiao N, Zhang Y, Manawasinghe IS, Li H, Xiong YR, Zhou T, Tan QL, Hoshino T, Lagaet P, De Lange R, Verbeken A, Tondeleir L, Manz C, Hampe F, Zhao CL, Yang Y, Zhang GQ, Dai DQ, Tomšovský M, Denchev TT, Denchev CM, Léveillé-Bourret É, Kemler M, Chen YP, Maharachchikumbura SSN, Feng ZX, Wang L, Wang QM, Wan SP, Yu FQ, Liu JK, Du HZ, Wu N, Hu HL, Su JY, Wang ZH, Hu YP, Yu H, Wang J, Yang YH, Dong W, Shu YX, Zhao HJ, Wang CQ, Xia WX, Liao CF, Doilom M, Li GJ, Suwannarach N, Senwanna C, Gafforov Y, Flakus A, Suchan T, Plata O, Rodriguez-Flakus P, Tamang J, Acharya K, Yang HD, Jayawardena RS, Tiendrebeogo A, Decock C, Somda I, Legrève A, Yang Y, Xiao YP, Zhou XZ, Li JX, Thongklang N, Han XX, Yang WQ, Wang SH, He MQ, Hyde KD, Zhao RL (2025) Fungal diversity notes 1919–2016: taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity 132: 425–651. https://doi.org/10.1007/s13225-025-00556-z
- Chomnunti P, Hongsanan S, Aguirre-Hudson B, Tian Q, Peršoh D, Dhami MK, Alias AS, Xu J, Liu X, Stadler M, Hyde KD (2014) The sooty moulds. Fungal Diversity 66(1): 1–36. https://doi.org/10.1007/s13225-014-0278-5
- Darriba D, Taboada GL, Doallo R, Posada D (2012) jModelTest 2: More models, new heuristics and parallel computing. Nature Methods 9(8): e772. https://doi.org/10.1038/nmeth.2109
- Ellis MB (1971) Dematiaceous hyphomycetes. Commonwealth Mycological Institute, Kew, 114–115. https://doi.org/10.1079/9780851986180.0000
- Ellis MB (1972) Dematiaceous hyphomycetes. XI. Mycological Papers 131: 1–25.
- Goh TK, Hyde KD, Tsui KM (1998) The hyphomycete genus Acrogenospora, with two new species and two new combinations. Mycological Research 102(11): 1309–1315. https://doi.org/10.1017/S0953756298006790
- Hall TA (1999) BioEdit: A User-Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98/NT. Nucleic Acids Symposium Series, 95–98.
- Harrington AH, Sarmiento C, Zalamea PC, Dalling JW, Davis AS, Arnold AE (2022) Acrogenospora terricola sp. nov., a fungal species associated with seeds of pioneer trees in the soil seed bank of a lowland forest in Panama. International Journal of Systematic and Evolutionary Microbiology 72: 005558. https://doi.org/10.1099/ijsem.0.005558
- Ho WH, Hyde KD, Hodgkiss IJ (2001) Fungal communities on submerged wood from streams in Brunei, Hong Kong, and Malaysia. Mycological Research 105(12): 1492–1501. https://doi.org/10.1017/S095375620100507X
- Hopple Jr JJ, Vilgalys R (1999) Phylogenetic relationships in the mushroom genus Coprinus and dark-spored allies based on sequence data from the nuclear gene coding for the large ribosomal subunit RNA: Divergent domains, outgroups, and monophyly. Molecular Phylogenetics and Evolution 13(1): 1–1. https://doi.org/10.1006/mpev.1999.0634
- Hu DM, Cai L, Chen H, Bahkali AH, Hyde KD (2010) Four new freshwater fungi associated with submerged wood from Southwest Asia. Sydowia 62: 191–203.
- Hughes SJ (1978) New Zealand Fungi 25. Miscellaneous species. New Zealand Journal of Botany 16(3): 311–370. https://doi.org/10.1080/0028825X.1978.10425143
- Hyde KD, Tennakoon DS, Jeewon R, Bhat DJ, Maharachchikumbura SSN, Rossi W, Leonardi M, Lee HB, Mun HY, Houbraken J, Nguyen TTT, Jeon SJ, Frisvad JC, Wanasinghe DN, Lűcking R, Aptroot A, Cáceres MES, Karunarathna SC, Hongsanan S, Phookamsak R, Silva NI, Thambugala KM, Jayawardena RS, Senanayake IC, Boonmee S, Chen J, Luo ZL, Phukhamsakda C, Pereira OL, Abreu VP, Rosado AWC, Bart B, Randrianjohany E, Hofstetter V, Gibertoni TB, Soares AMS, Plautz Jr HL, Sotão HMP, Xavier WKS, Bezerra JDP, Oliveira TGL, Souza-Motta CM, Magalhães OMC, Bundhun D, Harishchandra D, Manawasinghe IS, Dong W, Zhang SN, Bao DF, Samarakoon MC, Pem D, Karunarathna A, Lin CG, Yang J, Perera RH, Kumar V, Huang SK, Dayarathne MC, Ekanayaka AH, Jayasiri SC, Xiao Y, Konta S, Niskanen T, Liimatainen K, Dai YC, Ji XH, Tian XM, Mešić A, Singh SK, Phutthacharoen K, Cai L, Sorvongxay T, Thiyagaraja V, Norphanphoun C, Chaiwan N, Lu YZ, Jiang HB, Zhang JF, Abeywickrama PD, Aluthmuhandiram JVS, Brahmanage RS, Zeng M, Chethana T, Wei D, Réblová M, Fournier J, Nekvindová J, Barbosa RN, Santos JEF, Oliveira NT, Li GJ, Ertz D, Shang QJ, Phillips AJL, Kuo CH, Camporesi E, Bulgakov TS, Lumyong S, Jones EBG, Chomnunti P, Gentekaki E, Bungartz F, Zeng XY, Fryar S, Tkalčec Z, Liang J, Li G, Wen TC, Singh PN, Gafforov Y, Promputtha I, Yasanthika E, Goonasekara ID, Zhao RL, Zhao Q, Kirk PM, Liu JK, Yan JY, Mortimer PE, Xu J, Doilom M (2019) Fungal diversity notes 1036–1150: Taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 96(1): 1–242. https://doi.org/10.1007/s13225-019-00429-2
- Hyde KD, Norphanphoun C, Ma J, Yang HD, Zhang JY, Du TY, Gao Y, Gomes de Farias AR, Gui H, He SC, He YK, Li CJY, Liu XF, Lu L, Su HL, Tang X, Tian XG, Wang SY, Wei DP, Xu RF, Xu RJ, Yang Q, Yang YY, Zhang F, Zhang Q, Bahkali AH, Boonmee S, Chethana KWT, Jayawardena RS, Lu YZ, Karunarathna SC, Tibpromma S, Wang Y, Zhao Q (2023) Mycosphere notes 387–412 – novel species of fungal taxa from around the world. Mycosphere 14(1): 663–744. https://doi.org/10.5943/mycosphere/14/1/8
- Index Fungorum (2026) Index Fungorum. http://www.indexfungorum.org/Names/Names.asp [accessed on 28 February 2026]
- Jayasiri SC, Hyde KD, Jones EBG, Persoh D (2018) Taxonomic novelties of hysteri form Dothideomycetes. Mycosphere 9(4): 803–837. https://doi.org/10.5943/mycosphere/9/4/8
- Kirschner R (2025) A new cryptic species of the fungal genus Acrogenospora (Dothideomycetes, Ascomycota) from Taiwan. Taiwania 70: 125–131. https://doi.org/10.6165/tai.2025.70.125
- Krauss GJ, Solé M, Krauss G, Schlosser D, Wesenberg D, Bärlocher F (2011) Fungi in freshwaters: ecology, physiology and biochemical potential. FEMS Microbiology Reviews 35(4): 620–651. https://doi.org/10.1111/j.1574-6976.2011.00266.x
- Li L, Du HZ, Thiyagaraja V, Bhat DJ, Phookamsak R, Cheewangkoon R (2024) Two novel freshwater hyphomycetes, in Acrogenospora (Minutisphaerales, Dothideomycetes) and Conioscypha (Conioscyphales, Sordariomycetes) from Southwestern China. MycoKeys 101: 249–273. https://doi.org/10.3897/mycokeys.101.115209
- Liu NG, Hongsanan S, Yang J, Lin CG, Bhat DJ, Liu JK, Jumpathong J, Boonmee S, Hyde KD, Liu ZY (2017) Dendryphiella fasciculata sp. nov. and notes on other Dendryphiella species. Mycosphere: Journal of Fungal Biology 8(9): 1575–1586. https://doi.org/10.5943/mycosphere/8/9/12
- Liu SL, Zhao P, Cai L, Shen S, Wei HW, Na Q, Han MH, Wei RX, Ge YP, Ma HX, Karunarathna SC, Tibprommab C, Zhang B, Dai D, Lin L, Fan XL, Luo ZL, Shen HW, Lu L, Lu WH, Xu RF, Tohtirjap A, Wu F, Zhou LW (2025) Catalogue of fungi in China 1. New taxa of plant-inhabiting fungi. Mycology 16(1): 1–58. https://doi.org/10.1080/21501203.2024.2316066
- Liu YJ, Whelen S, Hall BD (1999) Phylogenetic relationships among ascomycetes: Evidence from an RNA polymerase II subunit. Molecular Biology and Evolution 16(12): 1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
- Ma J, Ma LG, Zhang YD, Xia JW, Zhang XG (2012) Acrogenospora hainanensis sp. nov. and new records of microfungi from southern China. Mycotaxon 120: 59–66. https://doi.org/10.5248/120.59
- Madeira F, Park YM, Lee J, Buso N, Gur T, Madhusoodanan N, Basutkar P, Tivey ARN, Potter SC, Finn RD, Lopez R (2019) The EMBL-EBI search and sequence analysis tools APIs in 2019. Nucleic Acids Research 47(W1): W636–W641. https://doi.org/10.1093/nar/gkz268
- Miller MA, Pfeiffer W, Schwartz T (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the 2010 gateway computing environments workshop (GCE), New Orleans, Louisiana, 7 pp. https://doi.org/10.1109/GCE.2010.5676129
- Rambaut A (2018) FigTree v1.4.4: Tree figure drawing tool. https://github.com/rambaut/figtree/releases
- Rehner SA, Buckley E (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-a sequences: Evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97(1): 84–98. https://doi.org/10.3852/mycologia.97.1.84
- Ronquist F, Teslenko M, Van Der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61(3): 539–542. https://doi.org/10.1093/sysbio/sys029
- Rossman AY, Crous PW, Hyde KD, Hawksworth DL, Aptroot A, Bezerra JL, Bhat JD, Boehm E, Braun U, Boonmee S, Camporesi E, Chomnunti P, Dai DQ, D'souza MJ, Dissanayake A, Gareth Jones EB, Groenewald JZ, Hernández-Restrepo M, Hongsanan S, Jaklitsch WM, Jayawardena R, Jing LW, Kirk PM, Lawrey JD, Mapook A, McKenzie EH, Monkai J, Phillips AJ, Phookamsak R, Raja HA, Seifert KA, Senanayake I, Slippers B, Suetrong S, Taylor JE, Thambugala KM, Tian Q, Tibpromma S, Wanasinghe DN, Wijayawardene NN, Wikee S, Woudenberg JH, Wu HX, Yan J, Yang T, Zhang Y (2015) Recommend ed names for pleomorphic genera in Dothideomycetes. IMA Fungus 6(2): 507–523. https://doi.org/10.5598/imafungus.2015.06.02.14
- Sivanesan A (1984) The Bitunicate Ascomycetes and their anamorphs. Strauss and Cramer GmbH. Hirschberg, 280–282.
- Stamatakis A, Alachiotis N (2010) Time and memory efficient likelihood-based tree searches on phylogenomic alignments with missing data. Bioinformatics 26(12): i132–i139. https://doi.org/10.1093/bioinformatics/btq205
- Swe A, Jeewon R, Pointing SB, Hyde KD (2009) Diversity and abundance of nematode-trapping fungi from decaying litter in terrestrial, freshwater and mangrove habitats. Biodiversity and Conservation 18: 1695–1714. https://doi.org/10.1007/s10531-008-9553-7
- Tan YP, Bishop HSL, Shivas RG, Cowan DA (2022) Fungal Planet description sheets: 1436–1477. Persoonia 49: 261–350. https://doi.org/10.3767/persoonia. 2022.49.08
- Wang WP, Hyde KD, Bao DF, Wanasinghe DN, Lin CG, Shen HW, Lu YZ, Zhang ZQ, Su XJ, Li YX, Al-Otibi F, Yang LQ, Luo ZL (2024) Lignicolous freshwater fungi from karst landscapes in Yunnan Province, China. Mycosphere 15(1): 6525–6640. https://doi.org/10.5943/mycosphere/15/1/29
- White TJ, Bruns TD, Lee SB, Taylor JW, Innis MA, Gelfand DH, Sninsky JJ (1990) Amplification and direct sequencing of Fungal Ribosomal RNA Genes for phylogenetics. PCR protocols: A guide to methods and applications (Vol. 18). Academic Press, New York, 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
- Xiao XJ, Liu NG, Liu JK, Shen HW, Yu YX, Yu XD, Xiao Y, Tian XG, Han MY, Bao DF, Liu JW, Yang Y, Ma J, Zhang JY, Sun YR, Luo ZL, Hongsanan S, Xiao YP, Jayawardena RS, Lu YZ, Sun JZ (2025) Fungal diversity of Ascomycota in China and Thailand: Descriptions of new taxa in honour of Kevin D. Hyde's 70th birthday. Mycosphere 16(2): 344–415. https://doi.org/10.5943/mycosphere/16/2/3
- Xu RJ, Hyde KD, Li JN, Boonmee S, Liu NG, Yang J, Li Y, Bao DF, Shen HW, Zhu XT, Zhu YA, Li TS, Xu K, Yu FM, Lu JR, Lei L, Wu N, Wu DM, Gao N, Jia PS, He XL, Al-Otibi F, Zhou DQ, Liu JK, Lu YZ, Luo ZL, Yang ZL, Zhao Q (2025) Lignicolous freshwater fungi of the pan Qinghai-Xizang Plateau, China. Fungal Diversity 133: 23–234. https://doi.org/10.1007/s13225-025-00555-0
- Yang J, Maharachchikumbura SSN, Liu JK, Hyde KD, Jones EBG, Al-Sadi AM, Liu ZY (2018) Pseudostanjehughesia aquitropica gen. et sp. nov. and Sporidesmium sensulato species from freshwater habitats. Mycological Progress 17(5): 591–616. https://doi.org/10.1007/s11557-017-1339-4
- Zhai ZJ, Yan JQ, Li WW, Gao Y, Hu HJ, Zhou JP, Song HY, Hu DM (2022) Three novel species of Distoseptispora (Distoseptisporaceae) isolated from bamboo in Jiangxi Province, China. MycoKeys 88: 35–54. https://doi.org/10.3897/mycokeys.88.79346
- Zhang D, Gao F, Jakovlić I, Zou H, Zhang J, Li WX, Wang GT (2020) PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Molecular Ecology Resources 20(1): 348–355. https://doi.org/10.1111/1755-0998.13096
- Zhang H, Zhu R, Qing Y, Yang H, Li C, Wang G, Zhang D, Ning P (2022) Polyphasic identification of Distoseptispora with six new species from fresh water. Journal of Fungi 8(10): e1063. https://doi.org/10.3390/jof8101063
- Zhu H, Cai L, Hyde KD, Zhang KQ (2005) A new species of Acrogenospora from submerged Bamboo in Yunnan, China. Mycotaxon 92: 383–386. https://doi.org/10.5962/p.414640