NSC-Reconstruct: Novel Strategies for Cell-based Neural Reconstruction
Published August 8, 2025 | Version v1
Dataset Open

NSCR_Dataset_2025

Authors/Creators

  • 1. Department of Biosciences, University of Milan, Milan 20133, Italy
  • 2. National Institute of Molecular Genetics "Romeo ed Enrica Invernizzi", Milan 20122, Italy
  • 3. Department of Neuroscience Rita Levi-Montalcini, University of Turin, Turin 10126, Italy
  • 4. Neuroscience Institute Cavalieri Ottolenghi, University of Turin, Orbassano 10043, Italy
  • 5. ROR icon University of Milan
  • 6. Neuroscience Institute Cavalieri Ottolenghi
  • 7. ROR icon University of Turin
  • 8. ROR icon Lund University
  • 9. ROR icon ETH Zurich
  • 10. University College London
  • 11. Carl Zeiss Spa, Research Microscopy Solution, Milan, Italy
  • 12. ROR icon Istituto Nazionale Genetica Molecolare
  • 13. ROR icon Fondazione IRCCS Istituto Neurologico Carlo Besta
  • 14. ROR icon University of Pavia
  • 15. ROR icon Vita-Salute San Raffaele University
  • 16. Università degli Studi di Milano

Description

The University of Turin (UniTo) released the third open-access dataset NSCR_Dataset collected for the NSC-Reconstruct (874758) European stem cell consortia for neural cell replacement, reprogramming and functional brain repair (874758)  (https://www.nsc-reconstruct.com/en/index.do).

This NSCR dataset contains the results from the following publication: https://www.sciencedirect.com/science/article/pii/S1043661825003305?via%3Dihub (corresponding authors: Prof. Annalisa Buffo, annalisa.buffo@unito.it; Prof. Elena Cattaneo, elena.cattaneo@unimi.it; Dr. Dario Besusso, dario.besusso@unimi.it). 

Results were obtained from histological ex vivo brain samples, in vivo calcium recordings and in vitro calcium imaging, ex vivo MEA recordings, and behavioral tests during chemogenetic stimulation from a rat model of Huntington's Disease (HD), after cell replacement approach of striatal progenitor cells in the lesioned striatum, at different time points. 

The dataset contains data from images of brain tissue stained for different cellular and molecular markers, acquired with confocal and lightsheet microscopes, from which analysis were performed, data from HD-MEA electrophisiological recordings of ex vivo brain slices containing the graft, data from calcium recordings perfomed in vivo, and behavioral data obtained eight month after transplantation by testing the HD animals in different tasks during chemogenetic modulation of graft activity.

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Additional details

Funding

European Commission
NSC-Reconstruct - Novel Strategies for Cell-based Neural Reconstruction 874758
Ministry of Education, Universities and Research
PRIN 2022 Prot. 2022LBENTH
Ministry of Education, Universities and Research
"Dipartimenti di Eccellenza” 2018–2022 and 2023–2027

References

  • Scaramuzza L, Ribodino M, Cassarino C, Morrocchi M, Gomez Gonzalez GB, Parolisi R, Sozzi E, Turrini G, Cerrato V, Conforti P, Hoxha E, Tognato R, Galeotti G, Cordiglieri C, Crosti MC, Zucca S, Lorenzati M, Bovetti S, Spaiardi P, de'Sperati C, Biella G, Ottoboni L, Parmar M, Maestri S, Besusso D, Cattaneo E, Buffo A. Transplanted human striatal progenitors exhibit functional integration and modulate host circuitry in a Huntington's disease animal model. Pharmacol Res. 2025 Sep;219:107905. doi: 10.1016/j.phrs.2025.107905. Epub 2025 Aug 10.