ASAP Data Repository
Published March 12, 2026 | Version 0.1
Dataset Open

Single nuclei RNA sequencing (ParseBio) of postmortem cingulate cortex,midbrain and motor cortex of healthy donors and Parkinson's disease patients.

Authors/Creators

  • 1. Laboratory of Computational Biology, VIB Center for AI & Computational Biology (VIB.AI), Leuven, Belgium,& VIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium.,& Department of Human Genetics, KU Leuven, Leuven, Belgium,& Laboratory of Integrative Cancer Genomics, VIB-KU Leuven Center for Cancer Biology, Leuven, Belgium,& Department of Oncology, KU Leuven, Leuven, Belgium
  • 2. Laboratory of Computational Biology, VIB Center for AI & Computational Biology (VIB.AI), Leuven, Belgium,& VIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium.,& Department of Human Genetics, KU Leuven, Leuven, Belgium
  • 3. Department of Human Genetics, KU Leuven, Leuven, Belgium,& KU Leuven Institute for Single Cell Omics (LISCO), University of Leuven, KU Leuven, Leuven, Belgium

Description

This Zenodo deposit contains a publicly available description of the Dataset:

Title: "Single nuclei RNA sequencing (ParseBio) of postmortem cingulate cortex,midbrain and motor cortex of healthy donors and Parkinson's disease patients.".

Description: This dataset consists of raw sequencing snRNA-seq data using ParseBio Evercode Whole Transcriptome. The data is part of an overall set of samples derived from postmortem midbrain (n=140), cingulate cortex (n=190) and motor cortex (n=4) of healthy donors (n=114), patients with Parkinson's disease (n=75) or patients with other neurological disorder (n=1). The protocol followed to isolate nuclei from postmortem brain samples and to prepare sequencing libraries can be found here: https://dx.doi.org/10.17504/protocols.io.5qpvo1rddg4o/v1, https://dx.doi.org/10.17504/protocols.io.8epv55xwdv1b/v1. To increase throughput and to decrease batch effects, several donors have been pooled together into a specific ParseBio barcode. To computationally demultiplex the nuclei to their corresponding donors, cellsnp-lite (version commit: aad18644adcde853c313362a856a24245c9b91f7) followed by vireo (https://github.com/single-cell-genetics/vireo/pull/108 ) has been used. The population VCF with the donor genotypes derived from whole genome sequencing data has been used to assign nuclei back to their donors.

This dataset is made available to researchers via the ASAP CRN Cloud: cloud.parkinsonsroadmap.org. Instructions for how to request access can be found in the User Manual.

This research was funded by the Aligning Science Across Parkinson's Collaborative Research Network (ASAP CRN), through the Michael J. Fox Foundation for Parkinson's Research (MJFF).

This Zenodo deposit was created by the ASAP CRN Cloud staff on behalf of the dataset authors. It provides a citable reference for a CRN Cloud Dataset

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Additional details

Funding

Aligning Science Across Parkinson's
Understanding inherited and acquired genetic variation in Parkinson’s disease through single-cell multi-omics analyses: a unique data resource ASAP-000430

References