BIO407 Group3: CLSM images of DFX treated cells

# REMBI metadata

## Study:

This data is part of the practical course BIO407 2026 at the University of Zurich, titled 'Advanced Microscopy: From preparation to data and visualization'.

The effects of Deferasirox (DFX) on mitochondria morphology were examined using different microscopy techniques.

## Study Component:

Job: Confocal microscopy
Task: 8 'Imaging Mitochondira in DFX-treated Cells'

Using confocal microscopy, images of DFX-treated and untreated cells were acquired.

## Biosample:

COS-7 cells (65536)

## Specimen:
Four different conditions:

-Control 1: No treatment

-Control 2: 5h DMSO treatment

-Condition 1: 3h 200uM DFX treatment
-Condition 2: 5h 200uM DFX treatment

Cells were fixed with 4% PFA, 0.05% GTA, 0.5% Saponin in PHEM.
Cells were labeled with DAPI, TOMM20-Alexa488, Tubulin-Alexa568, SiR-Actin.

## Image Acquisition:

Images were acquired using a "LeicaFALCON": Leica DMI6000B, Model SP8.

Acquired channels:

Channel 1: DAPI, 405nm excitation, 415nm-497nm emission

Channel 2: Alexa488-TOMM20, 488nm excitation, 497nm-548nm emission

Channel 3: Alexa568-Tubulin, 561nm excitation, 578nm-637nm emission

Channel 4: SiR-actin, 633nm excitation, 652nm-704nm emission

Objective: 63x HC PL APO CS2 oil objective, 1.4NA

## Image Data:

BIO407_260303_Group3_SP8_65536.lif/T8_63x_DMSO_1
BIO407_260303_Group3_SP8_65536.lif/T8_63x_DMSO_2
BIO407_260303_Group3_SP8_65536.lif/T8_63x_DMSO_overview
BIO407_260303_Group3_SP8_65536.lif/T8_63x_3h_DFX_1
BIO407_260303_Group3_SP8_65536.lif/T8_63x_3h_DFX_2
BIO407_260303_Group3_SP8_65536.lif/T8_63x_5h_DFX_1
BIO407_260303_Group3_SP8_65536.lif/T8_63x_no_treatment_1
BIO407_260303_Group3_SP8_65536.lif/T8_63x_no_treatment_overview