BRYOMOLECULES - D1.3 Inclusion of 40+ axenic liverwort accessions to the bryophyte culture collection

In this deliverable, we present 50 accessions of axenic liverworts which have been selected for
downstream analyses in the BRYOMOLECULES project, five of these recent additions (not yet
subject of RNA sequencing or chemical profiling). Plant material has been mostly collected from
Sweden, as well as from Iceland, The Faroe Islands and Italy. All samples were provided by LU.
The list includes collection information, such as sampling place, coordinates, date of sampling,
and collector names.
Sterile accessions have been achieved by surface sterilisation of either sporophytes or vegetative
plant material with a 0.05% solution of Sodium Dichloroisocyanurate for 1-2 minutes, followed
by washing with sterile deionised water. Accessions are maintained on Petri dishes with 1% agar
in a medium as described by Rudolph et al. (1988). The medium was 2 times the original
formulation with an addition of 12 mg of the fungicide Benomyl per litre to reduce the risk of fungal
contamination. If needed, samples have been treated to eliminate infections by cyanobacteria
using the antibiotic erythromycin. Collections are maintained in a culture room under artificial
LED-tube light, 16/8 day/night cycles and a mean temperature of 20° C.
The collection contains a wide range of liverworts, including both thallous species and leafy
liverworts. Taxon names are provided according to Hodgetts et al. (2020). Eventual synonyms,
mostly older names still commonly occurring in liverwort floras, are listed in a separate column.
The column “Source” indicates whether the accessions have been obtained from spore cultures
or from sterilized vegetative tissue. The column “RNA seq” indicates if the accession has been
included in transcriptomics analyses (see D3.1) and the column “Chemical” indicates if the
accession has been sent for phytochemical profiling (see D2.2). “Clade” and “Family” include
the taxonomic designation among liverworts, at order and family level, respectively. The last
column includes remarks pertinent to taxonomy or culturing.

References:

- Rudolph, H., Kirchhoff, M., Gliesmann, S. Sphagnum culture techniques. Proc. Methods in Bryology, Nichinan, 1988:25–34: Hattori Botanical Laboratory
- Hodgetts, N. G., L. Söderström, T. L. Blockeel, et al. “An Annotated Checklist of Bryophytes of Europe, Macaronesia and Cyprus.” Journal of Bryology 42, no. 1 (2020):1–116. https://doi.org/10.1080/03736687.2019.1694329.

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