Release v1.0.2

Combinatorial Promoter–RBS Expression Library

This release provides the fully annotated DNA sequences and formal SBOL3 specification of a combinatorial promoter–RBS expression library constructed in pSC101 and pGreen backbones.

Contents

• Annotated GenBank files for all plasmid constructs
• SBOL3 combinatorial derivations encoding the promoter × RBS design space
• Machine-readable metadata linking construct identifiers to biological parts
• Reproducibility scripts and automated validation workflow

Design Space

The library encodes combinations of:

• Constitutive sigma70 promoters (BioBrick J231xx family)
• Ribosome binding sites (B003x and J611xx series)
• GFPmut3 reporter
• Standard transcriptional terminator
• Two backbone contexts: pSC101 and pGreen

Reproducibility

The repository includes:

• scripts/make_sbol_library.py to regenerate SBOL3 derivations
• scripts/validate_library.py to validate GenBank annotations against metadata
• Continuous integration checks via GitHub Actions

Citation

Please cite the associated publication and this repository DOI (Zenodo).

SB2CL — Synthetic Biology and Biosystems Control Lab Universitat Politècnica de València