Data and Code for NCOMMS-25-57165A

this is the data and code depository for the paper "Generalization of fear learning is shaped by inhibitory sensory processing in mice", NCOMMS-25-57165.

miasma.zip ; contains custom-written imaging analysis software used in this article.
miasma_documentation.docx ; detailed description of files and analysis steps for miasma.
xls.zip ; contains the numerical data used in the findings. 
rstats.zip ; contains the stats codes written in rstudio.

description of files in xls:

- habituation.xls: dataset for habituation/dishabituation experiment.

- mobility.xls: dataset for mobility and mean calcium increase after drug application, in imaging experiments.
- all the animals are listed with their groups and mobility scores for each odor.
- sheet O includes OSN animals, sheet G for PG cells, sheet M for mitral. "PRD" isolates paired animals. ALL and ALL_SHORT has all the animals, latter in short format.

- openfield.xls: dataset for openfield experiments.
- detection: accuracy of ethovision, mobility: active time of the animals. center-zone2: percentage of time animals spent in each zone.
- dists: mean distance from each zone, all danger: areas adjacent to CS+., all safe: areas adjacent to no odor.
- sheet "testing_rep" includes interactable representative graphs for each point made in the paper along with additional ones.
- sheet "test_dist" isolated data of distances used to run stats.

- percentages.xls: dataset for calcium findings, change in df/f ater drug application.
- experimental specifics of all animals listed from B to E.
- odor: presented odor, roi: ROI number, max: change in df/f of peak fluorescence, maxtime: time of peak, time1-5: peak within time section, offvol/area: offset metrics.
- sheets are named according to cell type of animal. _rep includes interactable graphs for findings presented.

- peaks.xls: same as percentage, but divided by peaks rather than just percentages.
- block: 1, before application; 2, after application.

- spatial: dataset for spatial analysis.
- max_pre: peak before drug, max_post: peak after drug, perc: change in df/f 
- roi_x/y: centroid location of the ROI, ref_x/y: centroid location of the max responding CS+ glomerulus of specific animal.
- eucl: euclidian distance between the ROI and peak CS+ ROI, micron: pixel converted to microns based on lens used.
- overlap: 1 if this ROI is overlapping with any other, top50: 1 if this roi is a top 50 responding ROI.
- m400: 1 if ROI is within 400 microns of peak CS+ ROI.

description of files in rstats:

*all files include a comment for which excel file to run and what to change in order to get the specific subsection of the analysis.

- calcium_mobility_correlation: Fig 2K.
- compare_percentages: Fig 2 D-J.
- mobility_across_odors: Fig 1C.
- mobility_sex: Fig 1C, sex differences.
- no_ceiling: Fig S2.
- openf_distances: Fig 1D, Fig 4G-H.
- overlap_glom: Fig 3C-D.
- proximity_CS+: Fig 3E.
- habituation: Fig 5C-D.