Published February 27, 2026 | Version v1
Dataset Restricted

Dataset and R code for: Bacterial supplementation shapes honey bee gut microbiota and host metabolism under controlled and field conditions

  • 1. ROR icon Czech University of Life Sciences Prague
  • 1. Masaryk University, Faculty of Science
  • 2. ROR icon Masaryk University
  • 3. ROR icon Bee Research Institute
  • 4. ROR icon Czech University of Life Sciences Prague
  • 5. Institute of Animal Physiology and Genetics CAS Prague
  • 6. ROR icon Charles University
  • 7. Výzkumný ústav včelařský, s.r.o.
  • 8. Maynooth University

Description

This repository contains processed datasets, metadata, and scripts used to generate the figures and statistical analyses reported in the manuscript.

Raw sequencing and proteomics data are deposited in public repositories and referenced in the manuscript.

External repositories
• 16S rRNA sequencing data: NCBI Sequence Read Archive (SRA) under BioProject PRJNA1425762
• Proteomics data: PRIDE repository with the identifier PXD066488

Repository contents

1. NMR_RawData_MetaData.zip
   Contains processed 1H NMR spectral data and associated metadata used for metabolomic analysis.

2. Plots_16S.zip
   R scripts and processed data (corresponding to Supplementary Table S2) used to generate microbiome analysis figures and statistical analysis.

3. Plots_NMR.zip
   R scripts and processed data (corresponding to Supplementary Table S4) used for statistical analysis and visualization of NMR metabolomics data. 

4. Plots_Weight.zip
   Scripts and processed data (corresponding to Supplementary Table S1) used for statistical analysis and visualization of body and gut weight measurements.

Contact
For questions regarding the data or analysis pipeline, please contact:
Saetbyeol Lee (lees@af.czu.cz)

Files

Restricted

The record is publicly accessible, but files are restricted to users with access.

Additional details

Funding

Ministry of Agriculture
QK21010088
Ministry of Education Youth and Sports
LM2023064
Science Foundation Ireland
12/RI/2346(3)

Software

Programming language
R