Published February 20, 2026
| Version v1
Dataset
Open
Proteomic Profiling of Human Epileptic Brain Tissue
Authors/Creators
-
1.
Friedrich-Alexander-Universität Erlangen-Nürnberg
-
2.
Universitätsklinikum Erlangen
Description
This project describes the proteomic profiling of human epilepic brain tissue, alongside control samples. Microdissected hippocampal, neocortical, and white matter tissue was analyzed. The aim was to identify protein alterations associated for different epileptic conditions. Raw mass spectrometry data, along with search engine output, are included in this partial submission.
Sample Processing Protocol
Fresh-frozen brain tissue was homogenized in lysis buffer and beads. Protein extraction was done by adding lysis and digestion buffer with trypsin. Peptides were labeled with TMT-16-plex reagents for quantification. LC-MS/MS analysis was done by injecting peptides into Orbitrap Eclipse mass spectrometer and full-MS spectra were collected.
Data Processing Protocol
Raw MS files were processed with Proteome Discoverer 2.4 (Thermo Fisher Scientific). Spectra were matched against Homo Sapiens database obtained from UniProt. All results were filtered to a 1% FDR. Search engine output files and raw mass spectrometry files included.
Files
20240412_EVO_EVO_MVL_FAIMS_2CV_Endurance15_44min_P0705_DDA_TMTPro_Proteins.txt
Files
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Additional details
Identifiers
Related works
- Is published in
- Journal article: 10.3389/fnmol.2026.1728666 (DOI)
Funding
- Deutsche Forschungsgemeinschaft
- CRC 1540 EBM 460333672
Dates
- Available
-
2026-02-20
References
- Auer S, Hoffmann L, Schicht M, Blümcke I and Paulsen F (2026) Temporal lobe epilepsy is associated with neuroinflammation, extracellular matrix remodeling, and synaptic protein alterations. Front. Mol. Neurosci. 19:1728666. doi: 10.3389/fnmol.2026.1728666