Phase 0 State-Separability Validation Protocol (CAIS) A Cross-Laboratory Reproducibility Framework for Molecular–Electrochemical Signal Classification

This document does not attempt to prove “consciousness.”

It fixes a far narrower, experimentally answerable question:

Can controlled human state transitions 
(sleep, meditation, acute stress, selective anesthesia) 
produce statistically separable patterns in a molecular–electrochemical interface?

Phase 0 is not philosophical.
It is operational.

You do not need a new device.
You do not need a large cohort.
You do not need to adopt any ontology.

You need only:

• A time-series electrochemical signal (≥1 Hz recommended)
• A structured session: Baseline → Induction → Recovery
• Timestamped state labels
• A pre-declared feature extraction pipeline
• Cross-validated classification
• A decision rule (≥65% cross-validated accuracy)

If separability emerges, interface sensitivity is demonstrated.
If performance remains at chance level, a boundary condition is established.

Both outcomes are scientifically valuable.

The protocol is deliberately protective of participating investigators:

• Mandatory negative controls (artifact detection safeguard)
• Explicit leakage firewall rules
• Session-separated folds
• No post-hoc hyperparameter tuning
• Drift stabilization requirements
• Optional permutation testing for robustness
• Hash-lockable, replayable dataset structure (Engineering Compliance Annex)

This framework does not demand belief.
It demands measurement discipline.

Phase 0 tests structure only.
It does not claim interpretation.
It does not assert ontology.
It does not claim clinical meaning.

It asks one falsifiable question:
Is state-dependent signal clustering detectable under reproducible laboratory conditions?

This is a minimal-entry, lab-ready protocol.

If you have ever wondered whether physiological state transitions leave 
detectable molecular–electrochemical footprints — 
and you would prefer to test that boundary directly rather than debate it —

Phase 0 is your entry point.

Author: Jinho Lee, MD  
Salpida Institute of Consciousness Science (SICS)  
Seoul, Republic of Korea  
License: CC BY-SA 4.0