DEVELOPMENT OF AN ANALYTICAL METHOD FOR THE DETERMINATION OF INSULIN BY UV-SPECTROSCOPY IN PHARMACEUTICAL PREPARATIONS

ABSTRACT
A Simple, sensitive, specific, spectrophotometric method developed for the detection
of Insulin. Insulin was analysed by spectrophotometry. The optimum condition for the
analysis of the drug was established. The solvent selected for the study was 0.01N NaOH.
The λ max of the Insulin in the chosen solvent was found to be 235nm. The developed method
shows high sensitivity with linearity between 0.02 and 0.12IU/ml. The limit of detection and
the limit of quantification were found to be 0.0056IU/ml and 0.0170IU/ml, respectively. All
the calibration curves show a linear relationship between the absorbance and the
concentration, and the coefficient correlation was higher than 0.9947. The regression of the
curve was found to be Y=4.5005x. The precision of the method was found to be 93.19±1.78
against the label claim of 10IU/ml. The method has been validated according to ICH
guidelines and satisfactory results have been observed, which can be utilised for routine
analysis.