Notch4 Promotes Colorectal Cancer Cell Proliferation, Migration and Stemness via Activating the Notch Signaling Pathway

Notch4 expression in CRC cell lines (HCT116, SW480) and normal colonic epithelial cell line (NCM460) was detected by Western blot and qRT-PCR. Notch4 was overexpressed via plasmid transfection or knocked down via siRNA in HCT116 cells. Cell proliferation was assessed by CCK-8 assay, migration by scratch wound healing assay, stemness by sphere formation assay and expressions of Notch pathway-related proteins (NICD4, Hes1, Hey1) by Western blot.