Official Publication: Genomic Innovation in Precision Oncology – Integrated CRISPR-TTP Architecture for Ewing Sarcoma (Version 4.0)

Official technical specification and supplementary data for the peer-reviewed article: "Genomic Innovation in Precision Oncology: Integrated CRISPR-TTP Bioengineering Architecture for Ewing Sarcoma (Version 4.0)" published in Frontiers in Genetics (2026).

Abstract: This record contains the complete architectural blueprint and standardized operating procedures (SOP v2.0) for the Althea Framework. The architecture integrates high-fidelity CRISPR-Cas9 genome engineering, MR-guided Focused Ultrasound (FUS) triggered delivery, and AI-driven therapeutic orchestration.

Key Components Included:

1. Full Architectural Specification (v4.0): Detailed logic of the integrated bioengineering system.

2. Clinical Workflow: A 21-day GMP-ready pathway for autologous dendritic cell manufacturing and nanoparticle administration.

3. In Silico Validation Data: Predictive modeling results for tumor growth inhibition and immune response dynamics.

4. AI Co-pilot Framework: Methodology for real-time personalization and target optimization.

License: Released under CC BY 4.0. This work is intended to serve as an open-source survival infrastructure for treatment-resistant fusion-driven malignancies.