Selective autophagy acts as an immune rheostat to balance plant defense with cellular survival during viral infection - part II

The dataset contains all the original raw files for the supplemental figures to the following study  - and is a second version of the original preprinted work.

Marion Clavel^1,2*, Anita Bianchi^1,3, Roksolana Kobylinska¹, Roan Groh^1,4, Xi Zhang², Juncai Ma⁵, Ranjith K. Papareddy^1,3, Nenad Grujic¹, Lorenzo Picchianti^1,4, Ethan Stewart⁵, Michael Schutzbier¹, Karel Stejskal¹, Juan Carlos de la Concepcion¹, Victor Sanchez de Medina Hernandez^1,4,8, Yoav Voichek^1,9, Pieter Clauw¹, Joanna Gunis¹, Gerhard Durnberger¹, Jens Christian Muelders², Annett Grimm², Arthur Sedivy^6,10, Mathieu Erhardt⁷, Victoria Vyboishchikov¹, Peng Gao¹, Esther Lechner⁷, Emilie Vantard⁷, Thomas Potuschak⁷, Jakub Jez^6,11, Elisabeth Roitinger¹, Pascal Genschik⁷, Byung-Ho Kang⁵, Yasin Dagdas^1,3*

Affiliations:                                                                                                                 

¹Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter; Vienna, Austria.

²Max-Planck-Institut für Molekulare Pflanzenphysiologie; Potsdam-Golm, Germany.

³Heidelberg University, Centre for Organismal Studies (COS); 69120 Heidelberg, Germany.

⁴Vienna BioCenter PhD Program, Doctoral School of the University at Vienna and Medical University of Vienna; Vienna, Austria.

⁵School of Life Sciences, Centre for Cell & Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories; Hong Kong, China.

⁶Vienna Biocenter Core Facilities (VBCF); Vienna, Austria.

⁷ Institut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg; 12, rue du Général Zimmer, 67084 Strasbourg, France.

⁸Current affiliation: Department of Molecular and Cellular Biology, University of Geneva, Geneva, Switzerland

⁹Current affiliation: Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot 76100, Israel

¹⁰Current affiliation: BOKU University, Core Facility Biomolecular & Cellular Analysis, Vienna, Austria

¹¹Current affiliation: Australian Institute of Marine Science; PMB 3, Townsville MC, QLD, 4810, Australia

*Corresponding authors. Email: Marion Clavel (marion.clavel@mpimp-golm.mpg.de), Yasin Dagdas (yasin.dagdas@cos.uni-heidelberg.de)

Abstract: RNA viruses co-opt host endomembranes to form replication complexes, often triggering cellular stress and immune responses. Here, we show that Arabidopsis thaliana activates selective autophagy to respond to viruses targeting mitochondria, chloroplasts, and the endoplasmic reticulum. Rather than degrading viral components, autophagy selectively removes the immune regulator Enhanced Disease Susceptibility 1 (EDS1) to prevent cell death. This targeted mechanism is mediated by oligomeric metabolic enzymes that moonlight as selective autophagy receptors, linking organelle stress to immune homeostasis. Our findings establish selective autophagy as an essential immune rheostat, fine-tuning defense responses and safeguarding cellular integrity to promote host survival during viral infections.

One archive corresponds to one supplemental figure.