Published March 31, 2026 | Version v1

Comparing the performance of functional versus taxonomic metagenomics for detecting ammonia disturbances in the biogas system: functional and taxonomic annotation, gene counts and FAN concentrations

Authors/Creators

Description

The study archives contain the results of sequence processing. These files suffice for reproducing the statistical analysis.

Filename Content
[sample].emapper.annotations Functional annotations
CAT.ORF2LCA_incl_names.txt Taxonomic annotations
gene_counts.tsv Gene counts

As described in the publication, genes were predicted using Prodigal, and then functionally and taxonomically annotated using eggNOG-mapper 2 and CAT. Reads were aligned to genes using Bowtie 2, and then counted using featureCounts.

 

Free ammonia nitrogen (FAN) concentrations have been included to allow for reproducing the linear regularized regression. These concentrations have been statistically standardized. They are saved in the RDS format, used by R.

 

Detailed sample descriptions can be found in the European Nucleotide Archive under accession number PRJEB91257.

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