Published February 4, 2026 | Version v1
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ROOT EXTRACT OF Clerodendrum paniculatum L. AS A NATURAL HEPATOPROTECTIVE AGENT

Authors/Creators

  • 1. PG Scholar, Department of Pharmacology, Srinivas College of Pharmacy, Valachil, Mangalore – 574143, Karnataka, India.
  • 2. Associate Professor, Department of Pharmacology, Father Muller College of Pharmaceutical Sciences, Deralakatte, Mangalore – 575018, Karnataka, India.

Description

Objective: This study investigated whether the root extract of Clerodendrum paniculatum L. is effective as a hepatoprotective agent in rat models. Methods: The experiment utilized Wistar rats randomly assigned to five groups and treated with either paracetamol (2 g/kg, p.o.) or CCl₄ (0.7 ml/kg, i.p.) to induce hepatotoxicity. Test groups received Clerodendrum paniculatum root extract at doses of 200 mg/kg and 400 mg/kg, while a standard group received silymarin (25 mg/kg, p.o.). Liver function was assessed through biochemical markers— ALT, AST, ALP, and total bilirubin. Histopathological analysis of liver tissues was also performed. Results: Rats treated with the extract showed a significant, dose-dependent decrease in ALT, AST, ALP, and total bilirubin levels compared to toxic controls. The 400 mg/kg dose exhibited effects nearly comparable to silymarin. Histology confirmed reduced necrosis, inflammation, and fatty changes in liver tissue, indicating structural recovery. Preliminary phytochemical analysis revealed the presence of flavonoids, alkaloids, and tannins, supporting the extract’s antioxidant potential. Conclusion: Our results suggest that Clerodendrum paniculatum L. root extract offers substantial protection against liver toxicity, making it an effective natural agent against liver injury.

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References

  • 1. Gudadhe AN, Chakre SY, Vaidya PD, Mhala AP. A brief review on hepatoprotective drug. World J Pharm Pharm Sci., 2015; 4(4): 173-4.
  • 2. Ozougwu JC. Physiology of the liver. Int J Res Pharm Biosci, 2017; 4(8): 13-24.
  • 3. Gao B. Basic liver immunology. Cell Mol Immunol, 2016; 13(3): 265-6.
  • 4. Ilyas U, Katare DP, Aeri V, Naseef PP. A review on hepatoprotective and immunomodulatory herbal plants. Pharmacogn Rev., 2016; 10(19): 66-70.
  • 5. Delgado-Montemayor C, Cordero-Pérez P, Salazar-Aranda R, Waksman-Minsky N. Models of hepatoprotective activity assessment. Medicina Universitaria, 2015; 17(69): 222-8.
  • 6. Asrani SK, Devarbhavi H, Eaton J, Kamath PS. Burden of liver diseases in the world. J Hepatol, 2019; 70(1): 151-71.
  • 7. Busardò FP, Grieco A. Editorial - Drug-induced hepatotoxicity. Eur Rev Med Pharmacol Sci., 2017; 21(1 Suppl): 135-7.
  • 8. Amadi CN, Orisakwe OE. Herb-induced liver injuries in developing nations: An update. Toxics, 2018; 6(2): 24.
  • 9. Parimelazhagan T. Pharmacological assays of plant-based natural products. Springer International Publishing, 2016; p. 135-7.
  • 10. Lee AS, Persoff J, Lange SM. Liver function tests. Mayo Clinic Medical Manual, 2023: 373-87.
  • 11. Trefts E, Gannon M, Wasserman DH. The liver. Curr Biol., 2017; 27(21): R1147-51.
  • 12. Mao J, Tan L, Tian C, Wang W, Zhang H, Zhu Z, et al. Research progress on rodent models and its mechanisms of liver injury. Life Sci., 2024; 337: 122343.
  • 13. Devarbhavi H, Asrani SK, Arab JP, Nartey YA, Pose E, Kamath PS. Global burden of liver disease: 2023 update. J Hepatol, 2023; 79(2): 516-37.
  • 14. Thilagavathi R, Begum SS, Varatharaj SD, Balasubramaniam AK, George JS, Selvam C. Recent insights into the hepatoprotective potential of medicinal plants and plant-derived compounds. Phytother Res., 2023; 37(5): 2102-18.
  • 15. Gan C, Yuan Y, Shen H, Gao J, Kong X, Che Z, et al. Liver diseases: epidemiology, causes, trends and predictions. Signal Transduct Target Ther, 2025; 10(1): 33.
  • 16. Ambanna Natikar N, Venkaraddi Mangannavar C, Hanamantappa Shalavadi M, Suresh Kolli S. Hepatoprotective activity of Curcuma vamana ethanolic rhizome extract against paracetamol and CCl4 induced hepatotoxicity in albino rats. Rajiv Gandhi University of Health Sciences Journal of Pharmaceutical Sciences, 2020; 10(4).
  • 17. Islam Shawon S, Nargis Reyda R, Qais N. Medicinal herbs and their metabolites with biological potential to protect and combat liver toxicity and its disorders: A review. Heliyon, 2024; 10(3): e25340.
  • 18. Foghis M, Bungau SG, Bungau AF, Vesa CM, Purza AL, Tarce AG, et al. Plants-based medicine implication in the evolution of chronic liver diseases. Biomed Pharmacother, 2023; 158: 114207.
  • 19. Al-Snafi AE, Mousa HN, Majid WJ. Medicinal plants possessed hepatoprotective activity. IOSR J Pharm, 2019; 9(8): 26-56.
  • 20. Ali SA, Sharief NH, Mohamed YS. Hepatoprotective activity of some medicinal plants in sudan. Evid Based Complement Alternat Med., 2019; 2019: 1-16.
  • 21. Mancak M, Altintas D, Balaban Y, Caliskan UK. Evidence-based herbal treatments in liver diseases. Hepatology forum, 2024; 5(1): 50-60.
  • 22. Kopilakkal R, Chanda K, Balamurali MM. Hepatoprotective and antioxidant capacity of Clerodendrum paniculatum flower extracts against carbon tetrachloride-induced hepatotoxicity in rats. ACS Omega, 2021; 6(40): 26489-98.
  • 23. Vinutha B, Lakshman K. In-vitro hepatoprotective activity of Pagoda (Clerodendrum paniculatum) leaves. Int J Pharm Res Appl., 2022; 7(4): 2456-4494.
  • 24. Kekuda TRP, Sudharshan SJ. Ethnobotanical uses, phytochemistry and biological activities of Clerodendrum paniculatum L. (Lamiaceae): A comprehensive review. J Drug Deliv Ther, 2018; 8(5-s): 28-34.
  • 25. Parmar YM. Hepatoprotective effect of Amomum subulatum Roxb seeds on carbon tetrachloride-induced liver damage in rats. IOSR J Pharm, 2012; 2(5): 38-43.
  • 26. Phuneerub P, Limpanasithikul W, Palanuvej C, Ruangrungsi N. In vitro anti-inflammatory, mutagenic and antimutagenic activities of ethanolic extract of Clerodendrum paniculatum root. J Adv Pharm Technol Res., 2015; 6(2): 48-52.
  • 27. Shaikh JR, Patil M. Qualitative tests for preliminary phytochemical screening: An overview. Int J Chem Stud., 2020; 8(2): 603-8.
  • 28. Mir SA, Mishra AK, Reshi ZA, Sharma MP. Preliminary phytochemical screening of some pteridophytes from district Shopian (J & K). Int J Pharm Pharm Sci., 2014; 5(Suppl 4): 632–7.
  • 29. D VB, Kulkarni R, Bhatt R. Evaluation of Hepatoprotective activity of Jasminum Sambac in rats. Int J Res Pharmacol Pharmacother, 2017; 6(2): 104-16.
  • 30. Jacob JM, Olaleye MT, Olugbuyiro JAO. Hepatoprotective effect of Alchornea cordifolia leaf on liver damage in albino rats. Int J Appl Sci Biotechnol, 2014; 2(2): 217-21.
  • 31. Saha P, Talukdar AD, Nath R, et al. Role of natural phenolics in hepatoprotection: a mechanistic review and analysis of regulatory network of associated genes. Front Pharmacol, 2019; 10: 509.
  • 32. Mamuaja MN, Herlina T, Rumampuk RJ, Maksum IP, Rukayadi Y. Flavonoids from Clerodendrum genus and their biological activities. ADMET DMPK, 2024; 12(6): 843-79.
  • 33. Ramachandran A, Jaeschke H. Mechanisms of acetaminophen hepatotoxicity and their translation to the human pathophysiology. J Clin Transl Res., 2017; 3(Suppl 1): 157-169.
  • 34. Al Amin ASM, Menezes RG. Carbon tetrachloride toxicity. In: StatPearls. Treasure Island (FL): StatPearls Publishing, 2023.
  • 35. Thakur S, Kumar V, Das R, Sharma V, Mehta DK. Biomarkers of hepatic toxicity: an overview. Curr Ther Res., 2024; 100: 100737.
  • 36. Gillessen A, Angelico F, Chen J, Lu L, Lucena MI, Fu Q, et al. Silymarin for treating toxic liver disease: international consensus recommendations. Gastro Hep Adv, 2022; 1(5): 882-93.