Published February 2, 2026 | Version v1
Software Open

Supplementary material and data for FloBuzz: A modular feeder system for automated aversive conditioning in bees

Authors/Creators

  • 1. ROR icon Middle East Technical University
  • 2. ROR icon Ankara University

Description

Supplementary material and data for publication: Gultekinoglu E., Erdem, B., Botasun, A., Arslan, O. C., Sevin, S., Gozen, A. G., Alemdar, H., Sahin, E., & Turgut, A. E. (2026). FloBuzz: a modular feeder system for automated aversive conditioning in bees. bioRxiv. https://doi.org/10.64898/2026.02.08.704633

 
inTrigger_Codes.zip includes the scripts of the inTrigger module:
- In_Trigger_GUI.py is the user interface of the In-Trigger module.
- In_Trigger_Core.py is the core machine code of the In-Trigger module.
- In_Trigger_Arduino.ino is code for the microprocessor of the shock switch.
- In_Trigger_ReadMe is the information and usage manual of the In-Trigger module.
- ShockSwitchDiagram.pdf is the circuit diagram to build the shock switch.
 
Feeder_CAD_Files.zip includes the printable 3D components of the feeder:
- Cap.stl is the STL file for the cap of the feeder module.
- CollectingBox.stl is the STL file for the collecting box of the feeder module.
- ElectricShockGrid.stl is the STL file for the electric shock grid of the feeder module.
- MainBase.stl is the STL file for the main base of the feeder module. 
- FloBuzzTestSetup.f3z is the assembled Fusion 360 file of the complete experimental setup, provided as a representative system-level configuration.
 

SyringePump_Codes.zip includes the necessary files to build the pump control unit.
- SyringePump.ino contains the firmware running on the microcontroller of the pump control unit.
- SyringePumpLogger.py is used to record operational logs generated by the syringe pump during runtime.
- ReadMe.txt describes the basic features of the syringe pump and provides usage instructions.
- PumpControlUnitPCB.jpg shows the circuit diagram of the custom-designed PCB for the pump control unit.


FloBuzz_Stat.zip includes statistics scripts and experimental data.
- FloBuzz_statistics.Rdm is a statistical analysis script to evaluate experimental data in the R language.
- FloBuzz_data.xlsx includes experimental data. The data was prepared by manually scoring the 450-second video recording of the electric shock-avoidance experiment at 5-second intervals. The number of bees on the flower and whether the electric shock was active were noted. 

Files

Feeder_CAD_Files.zip

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Additional details

Related works

Is supplement to
Preprint: 10.64898/2026.02.08.704633 (DOI)