FIGURE 5. Transverse sections of the phylloclade of Phyllanthus monteazulensis, A and B. The epidermis has some papillose cells (A, inset), the thick cuticle and outer epidermal cell wall (orange color) as evidenced by Sudan IV staining, followed by the chlorenchyma with 2–3 layers, and fibers with very thick pectin-rich walls highlighted by ruthenium red, C–E. Detail of the papillae (part of the walls stained purplish-pink) and the subepidermal layers containing cells with phenolic compounds (green coloration), evidenced by toluidine blue, F and G. Details of the epidermis showing the pectic portion of the cell wall forming projections between the lipidic layers of the wall, extending towards the cuticle (arrowheads) after staining with ruthenium red (F) and Sudan IV (G), H and I. Druse-type crystals (arrows; H inset) and fibers, observed under polarized light, are present throughout the phylloclade,. Ct = cuticle; Ep = epidermis; Fi = fibers; Pc = chlorenchyma; Stomata = St; Xy = xylem. Images by Beatriz Appezzato-da-Glória & Marli Kasue Misaki Soares. All images from D.A. Zavatin et al. 2021 specimen.