Published January 28, 2026 | Version v1
Image Open

TIRFM images of human primary CD8+ T cells on supported lipid bilayer

Authors/Creators

  • 1. ROR icon Oslo University Hospital
  • 2. ROR icon University of Oxford
  • 3. Kennedy Institute of Rheumatology, Nuffield Department of Orthopedics, Rheumatology and Musculoskeletal Sciences, and Chinese Academy of Medical Sciences Oxford Institute, Nuffield Department of Medicine, University of Oxford

Description

Raw TIRFM images of primary human CD8+ T cells activated on supported lipid bilayers functionalized with ICAM-1, UCHT-1, and CD58. TIRFM imaging was performed with an Olympus IX83 inverted microscope (Keymed, Southend-on-Sea, UK) equipped with 405 nm, 488 nm, 561 nm and 640 nm laser lines, an Andor sCMOS Prime 95B camera and a 100x 1.45 NA oil immersion objective.

The imaging channels are as follows:

  • 405: ICAM-1
  • 488: UCHT1
  • 568: Phalloidin/Actin
  • 647: CD58
  • SIRC
  • Brightfield (BF)

A detailed description can be found in the published manuscript. DOI:  https://doi.org/10.3389/fimmu.2026.1746651

Files

Test_data.zip

Files (182.7 MB)

Name Size Download all
md5:25e033b5dd39df02fecd08d66e496e39
182.7 MB Preview Download

Additional details

Related works

Is described by
Journal article: 10.3389/fimmu.2026.1746651 (DOI)