Published January 28, 2026 | Version v1
Dataset Open

Chromosome-scale and nuclear haplotype-phased assemblies of Puccinia striiformis f. sp. tritici isolates Pst239E (PstS10) and PstS7

Authors/Creators

  • 1. ROR icon Aarhus University
  • 2. Imperial College London
  • 3. ROR icon University of Oxford
  • 4. International Center for Agricultural Research in the Dry Areas
  • 5. ROR icon University of Tasmania
  • 6. ROR icon Australian National University
  • 7. ROR icon Instituto Nacional de Investigación Agropecuaria
  • 8. Aarhus Universitet

Description

Chromosome-scale and nuclear haplotype-phased assemblies of two Puccinia striiformis f. sp. tritici isolates, Pst239E (PstS10) and PstS7

The fully haplotype-phased reference genome for an Australian sampled Pst239E isolate, which belongs to the PstS10 lineage, was generated using PacBio HiFi and Oxford Nanopore Technology (ONT) R10.4-based long-reads and Hi-C as input for Verkko hybrid genome assembler (Rautiainen et al., 2023). The haplotype-phased reference genome of PstS7 used ONT R10.4.1 simplex reads and Hi-C with hifiasm --ont (Cheng et al., 2025). Please see the assembly quality statistics table in the manuscript. 

The assemblies have also been submitted to NCBI with the following accession, waiting to be processed and released. 

Pst239E (PstS10)

  • PRJNA1260730 : Puccinia striiformis f. sp. tritici haplotype 1 genome sequencing  (haplotype A)
  • PRJNA1260729 : Puccinia striiformis f. sp. tritici haplotype 2 genome sequencing  (haplotype B)

PstS7

  • PRJNA1260732 : Puccinia striiformis f. sp. tritici haplotype 1 genome sequencing  (haplotype A)
  • PRJNA1260731 : Puccinia striiformis f. sp. tritici haplotype 2 genome sequencing  (haplotype B)

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