JNM Lab
Published January 27, 2026 | Version v1
Conference paper Restricted

Towards tracing lipid-nanoparticle intracellular trafficking in cancer cells by confocal Raman microscopy (Conference Abstract)

Authors/Creators

  • 1. Centre for Innovative Biomedicine and Biotechnology
  • 2. ROR icon University of Coimbra
  • 3. ROR icon Instituto de Biomedicina de Sevilla
  • 4. Faculty of Pharmacy, University of Coimbra
  • 5. Center for Neurosciences and Cell Biology, University of Coimbra

Description

Lipid nanoparticles (LNPs) can enhance the efficacy of mRNA-based cancer therapy by increasing bioavailability upon preserving mRNA integrity and stability until they reach their intended targets. However, the success and safety of LNPs is largely dependent on their intracellular trafficking. Confocal Raman microscopy (CRM) offers insights into the unique spectral fingerprint of samples with subcellular resolution and can shed light on the cell uptake and transport of LNPs. In this preliminary study, we investigate the potential of this imaging modality to track LNPs in live cancer cells. 

Files

Restricted

The record is publicly accessible, but files are restricted. <a href="https://zenodo.org/account/settings/login?next=https://zenodo.org/records/18386199">Log in</a> to check if you have access.

Additional details

Funding

European Commission
CInTech NextGenerationEU - Portuguese Resilience and Recovery Plan (PRR-30): C644865576-00000005