Published January 24, 2026 | Version v1
Dataset Open

FLIM of human FoxP1 wildtype and mutants - part III

Authors/Creators

  • 1. ROR icon University of Würzburg

Contributors

Project leader:

Project manager:

  • 1. ROR icon University of Würzburg
  • 2. ROR icon University of Chile
  • 3. ROR icon Clemson University

Description

Part I: 10.5281/zenodo.18347876

Part II: 10.5281/zenodo.18360348

Part III: 10.5281/zenodo.18362153

This dataset is one out of three zenodo entries encompassing a PIE-FLIM dataset of
full-length human FoxP1, point-mutation and truncation variants acquired in live HEK293T cells.

This dataset contains
(i) the Python script used to analyse the data
(ii) the raw data (Picoquant .ptu format)
(iii) binary masks used to the select the region of interest

All other data (e.g. fluorescence intensity decays), sub-masks and values can be created from 
the raw data and the masks using the provided scripts.

A detailed description of how to install the required packages for the scripts and how to run them
was published in a similar project:

10.5281/zenodo.17869433


The 3 zenodo entries contain the following datasets:

Part I:

khm_20231117_FoxP: Full-length, wildtype FoxP1
khm_20231201_FoxP: ZIP-FKH truncation variant wildtype of FoxP1 or including R514H mutation
khm_20231208_FoxP: ZIP-FKH truncation variant wildtype of FoxP1 or including the delta D358 deletion
khm_20240308_FoxP: Full-length, wildtype FoxP1 with R514H mutation

Part II:

khm_20231213_FoxP: ZIP-FKH truncation variant of FoxP1 including the delta D358 deletion or the R514H mutation
khm_20231124_FoxP: Full-length, wildtype FoxP1 and including the delta D358 deletion
khm_20231129_FoxP: Full-length, wildtype FoxP1 including the delta D358 deletion or the R514H mutation
khm_20231220_FoxP: FKH truncation variant of FoxP1 wildtype or including R514H mutation 

Part III:

khm_20231215_FoxP: FKH truncation variant of FoxP1 wildtype or including R514H mutation 

Files

khm_20231215_FoxP.zip

Files (19.7 GB)

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Additional details

Dates

Collected
2023-11-17/2024-03-08