ELISA-based protein-PAMP interaction assay

The interaction between HMC or metHb with LPS or LTA are examined by ELISA using LPS- or LTA-immobilized plates and probed with anti-hemocyanin or anti-hemoglobin antibody. To test the specificity of the ELISA, we further examined whether or not pre-incubation of HMC or Hb with LPS or LTA could reduce the ELISA-readout in a dose-dependent manner.