Published December 16, 2025
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TABLE 2 in Unravelling a Taxonomic Puzzle: Reinstating the South African Scolelepis capensis as distinct from Scolelepis squamata (Annelida: Spionidae)
Authors/Creators
- 1. Department of Botany and Zoology, Stellenbosch University, Stellenbosch, 7600, South Africa
- 2. Department of Conservation and Marine Science, Cape Peninsula University of Technology, Cape Town, 8000, South Africa
Description
TABLE 2. PCR Thermal cycling conditions for three genes: COI, 18S and 28S used to amplify Scolelepis specimens from South Africa, adapted from Surugiu et al. (2022).
| No. of cycles | |||||
|---|---|---|---|---|---|
| Gene | Initial denaturation | Denaturation | Annealing | Extension | Final Extension |
| COI | 38 | ||||
| 94°C—3 mins | 94°C—30s | 46°C—45s | 72°C—1min | 72°C—5min | |
| 18S | 40 | ||||
| 94°C—3 mins | 94°C—30s | 51°C—45s | 72°C—1.5min | 72°C—5min | |
| 28S | 40 | ||||
| 94°C—5 mins | 94°C—20s | 52°C—45s | 72°C—1.5min | 72°C—5min | |
Notes
Published as part of Smith, Lee Vernon, Kara, Jyothi & Simon, Carol Anne, 2025, Unravelling a Taxonomic Puzzle: Reinstating the South African Scolelepis capensis as distinct from Scolelepis squamata (Annelida: Spionidae), pp. 404 in Zootaxa 5729 (3) on page 404, DOI: 10.11646/zootaxa.5729.3.1, http://zenodo.org/record/18018740
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Related works
- Is part of
- Journal article: 10.11646/zootaxa.5729.3.1 (DOI)
- Journal article: urn:lsid:plazi.org:pub:A553FD7A1A553D2D936CFFE68511FFC0 (LSID)
- Journal article: http://publication.plazi.org/id/A553FD7A1A553D2D936CFFE68511FFC0 (URL)
- Journal article: http://zenodo.org/record/18018740 (URL)
- Journal article: http://zoobank.org/6BC0B4F4-A3F6-4FAD-959C-42D92DEDF8DC (URL)
References
- Surugiu, V., Schwenter, M. & Meissner, K. (2022) Fixing the identity of Scolelepis squamata (Annelida: Spionidae) - neotype designation, redescription and DNA barcode sequences. Systematics and Biodiversity, 20 (1), 1-25. https://doi.org/10.1080/14772000.2021.2003906