nf-core/ampliseq: Ampliseq Version 2.16.0

nf-core/ampliseq version 2.16.0 - 2026-01-16

Summary of changes

Updates of tools and databases, and reductions in resource allocations. Resources should be now much more efficiently used, but for extraordinary large data resource allocations could be too low, please give feedback and a solution will be found!

Detailed changes

Changed

• #928 - Resource allocations were reduced for most smaller processes.
• #931 - For --dada_ref_taxonomy, replace sbdi-gtdb=R10-RS226-1 with updated database sbdi-gtdb=R10-RS226-2 (see https://figshare.scilifelab.se/articles/dataset/SBDI_Sativa_curated_16S_GTDB_database/14869077/10)

Fixed

• #926,932 - Template update for nf-core/tools version 3.5.1
• #929,#935 - A bug in a dependency of MultiQC can lead (rarely) to plot generation being omitted, without warning. In that case, the subsequent pipeline summary report failed previously, now it gracefully handles that issue.

Dependencies

• #936,#940 - Updated some software versions

| software | previously | now | | -------- | ---------- | --------- | | nextflow | >=25.04.0 | >=25.04.8 | | Cutadapt | 4.6 | 5.2 | | DADA2 | 1.30.0 | 1.34.0 | | Phyloseq | 1.46.0 | 1.50.0 | | MultiQC | 1.29 | 1.33 |